No abstract

Wheat peptides, the biological active peptides derived from foods, has an array of biological actions, including antiobesity, antimicrobial, and angiotensin I-converting enzyme inhibitory effects in mammalian species. Recent studies showed that some wheat peptides may show the noteworthy antioxidant potency against the peroxidation of lipids or fatty acids, but the effect of oxidation on its antioxidant activities is unclear. In the present study, we demonstrate that heat and malandialdehyde (MDA)-oxidized wheat peptides lose its surface hydrophobicity and reducing power, and show a relatively lower free radical-scavenging activitiy in vitro. Those modifications also lead to gradual formation of aggregates in wheat peptides and induce more reactive oxygen species (ROS) production in vivo. These findings indicate that oxidation may influence the functional properties and directly alter the structure of wheat peptides, and lead to the loss of its antioxidant potency both in vitro and in vivo, thereby providing a novel explanation for some of the potential health risks proposed for oxidized food in human.

Oral dosage forms have many obvious advantages like simple, painless and self-administered. While the bioavailability of drugs are always low for they are unstable and usually degraded within gastrointestinal tract. Curcumin, a multifunction native compound, can be used in curing many diseases while the oral bioavailability is relatively low, limiting its clinical effectiveness. The aim of this study was to investigate the method of increasing the bioavailability by the addition of biotin, which was a kind of vitamin helping the liposomes to uptake by biotin receptors. In situ rat Single-Pass Intestinal Perfusion Model (SPIP) was applied to investigate the different content of biotin and caco-2 cells monolayer model was established to mimic the intestinal tract in drug transportation study. As the results shows that biotin decorated liposomes (biotin-PEG-DSPE-Lip) had a better cellular uptake and transportation rate compared to biotin undecorated ones (PEG-DSPE-Lip). It indicated that the bioavailability of curcumin had been increased.