The mathematical model of optimal daily operation for multiple pump units with adjustable-blade in single pumping station was solved by means of decomposition-dynamic programming aggregation method and experimental optimization method while the peak-valley electricity price was taken into consideration. After taking No.4 Huaian Pumping Station as a study case, a series of optimal operation schemes under different average daily heads and water pumping loads were obtained. Based on the analysis of optimization benefits, the applicability comparison of two optimal algorithms have been carried out, by which the application scope of each algorithm was proposed.