This paper presents a systematic method for calculating regional level Environmental sustainability by using the sustainable value approach. The quest for achieving sustainability has led to the development of various tools and measures for structuring and conducting sustainable development policy analyses and the metrics used for the measurement of sustainability are still evolving. Sustainable Value approach is another simple tool for measuring sustainability performance. In general, Sustainable Value integrates the three dimensions of sustainability: the economic development, environmental sustainability and social development. This paper uses the principles of the sustainable value approach to calculate the environmental sustainability of the regions of the Czech Republic.