We present an advanced velocity model building and imaging methodology that resulted in significant enhancements in defining the salt flanks with overhangs and subsalt structures. The key technologies used were (a) True Azimuth Multiple Elimination (TAME), (b) Tilted Transverse Isotropy (TTI) model building (FAN), (c) TTI Reverse Time Migration (RTM), (d) RTM Delayed Imaging Time (DIT) scans and (e) post-migration multiple attenuation. Approximately 39 OCS blocks of the Lena narrow-azimuth survey located in the Mississippi Canyon area of the Gulf of Mexico were imaged. The area was previously imaged with the Vertical Transverse Isotropy (VTI) Kirchhoff migration. Significant improvements have been achieved by reprocessing the same data with the new imaging methodology.
Infections due to methicillin-resistant Staphylococcus aureus (MRSA) are present worldwide and represent a major public health concern. The capability of PCR followed by high-resolution melt (HRM) curve analysis for the detection of community-associated and livestock-associated MRSA strains and the identification of staphylococcal protein A (spa) locus was evaluated in 74 MRSA samples which were isolated from the environment, humans, and pigs on a single piggery. PCR-HRM curve analysis identified four spa types among MRSA samples and differentiated MRSA strains accordingly. A nonsubjective differentiation model was developed according to genetic confidence percentage values produced by tested samples, which did not require visual interpretation of HRM curve results. The test was carried out at different settings, and result data were reanalyzed and confirmed with DNA sequencing. PCR-HRM curve analysis proved to be a robust and reliable test for spa typing and can be used as a tool in epidemiological studies.
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