Boys with microtia faced more psychological problems than girls. Their mental disorders apparently increased when they reached adulthood. The mothers of microtia patients endured more mental problems. Thus, this study provided a better understanding of the psychosocial morbidity experienced by microtia patients and their parents.

Identifying urban vitality in large cities is critical for optimizing the urban fabric. While great attention has been paid to urban vitality in developed countries, related studies have been rarely conducted in developing countries. In this study, we defined urban vitality as the capacity of an urban built environment to boost lively social activities and developed a framework for measuring urban vitality using the dimensions of built environment, human activities, and human-environment interaction. Taking Shanghai, China as a case, we conducted a measurement of urban vitality using multi-source data. The results show that Shanghai follows a monocentric vital pattern within the outer ring road, with urban vitality declining from the central urban core to the city periphery. While the old urban cores tend to show high urban vitality, Pudong New Area is mostly dominated by low vitality. Three clusters with high urban vitality were identified: the old urban area, the Lujiazui CBD, and residential agglomeration areas. We conducted validation of the measuring results using phone usage density. Urban vitality showed a positive correlation with phone usage density, indicating a high accuracy of assessment. We also discovered that European-style block planning, zoning plan, mixed-functional development, urban renewal regulation, and migrant concentration were playing leading role in urban vitality of Shanghai.

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Hair follicle stem cells (HFSCs) possess powerful expansion and multi‑differentiation potential, properties that place them at the forefront of the field of tissue engineering and stem cell‑based therapy. The aim of the present study was to investigate the differentiation of human HFSCs (hHFSCs) into cells of an endothelial lineage. hHFSCs were expanded to the second passage in vitro and then induced by the addition of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) to the culture medium. The expression levels of endothelial cell (EC)‑related markers, including von Willebrand factor (vWF), vascular endothelial cadherin (VE)‑cadherin and cluster of differentiation (CD)31, were detected by immunofluorescence staining, flow cytometric analysis and reverse transcription‑polymerase chain reaction. The hHFSCs expressed vWF, VE‑cadherin and CD31 when exposed to a differentiation medium, similar to the markers expressed by the human umbilical vein ECs. More significantly, differentiated cells were also able to take up low‑density lipoprotein. The data of the present study demonstrated that an efficient strategy may be developed for differentiating hHFSCs into ECs by stimulation with VEGF and bFGF. Thus, hHFSCs represent a novel cell source for vascular tissue engineering and studies regarding the treatment of various forms of ischaemic vascular disease.