Background. Healthy foods have been met with marked success in the last two decades. Lupine fl ours, protein concentrates, and isolates can be applied as a substance for enriching different kinds of food systems such as bakery products, lupine pasta, ice cream, milk substitutes. Imitation processed cheese is made from mixtures of dairy and/or non dairy proteins and fat/oils and is variously labeled analogue, artifi cial, extruded, synthetic and/or fi lled. Processed cheese can be formulated using different types of cheese with different degree of maturation, fl avorings, emulsifying, salts, and/or several ingredients of non-dairy components. Non-dairy ingredients have been used in processed cheese for many dietary and economic reasons. In this study, lupine paste was used to substitute 25, 50, 75 and 100% of cheese in base formula of processed cheese analogue (PCA). Material and methods. Matured Ras cheese (3 months old) was manufactured using fresh cow milk. Soft cheese curd was manufactured using fresh buffalo skim milk. Emulsifying salts S9s and Unsalted butter were used. Lupine termis paste was prepared by soaking the seeds in tap water for week with changing the water daily, and then boiled in water for 2 hrs, cooled and peeled. The peeled seeds were minced, blended to get very fi ne paste and kept frozen until used. Results. Lupine paste was used to substitute 25, 50, 75 and 100% of cheese in base formula of processed cheese analogue (PCA). The obtained PCA were analysed when fresh and during storage up to 3 months at 5 ±2°C for chemical composition, physical and sensory properties. The histopathological effect of lupines on alloxan diabetic albino rats and nutritional parameters were also investigated. Incorporation of lupine paste in PCA increased the ash and protein contents while meltability and penetration values of resultant products were decreased. Adding lupine in PSA formula had relatively increased the oil index and fi rmness of products. Feeding rats a balanced diet containing processed cheese enriched with lupine showed marked improvements in islets structure and lowered blood glucose compared to rats fed on basil diet (negative group). Springiness was greatly reduced with increasing the added ratio of lupine in the formula of cheese. All processed cheese produced were sensory acceptable but an overall acceptability was lowered by incorporating lupine in PCA formula. Body and texture score of PCA was the mostly affected by increasing lupine ratio in formula without signifi cant difference up to 50% substitution of cheese base.

Domiati cheese was made from heated cow's milk with tow levels of salt (5% or 10%) with adding 1% activated commercial starter cultures of YC-X11 (Str. thermophilus and Lb. delbruekii ss. bulgaricus, T1), Bio Profit (Lb rhamnosus and Propio. freudenreichii ss. shermanii, T2) and LC 705 (Lb. casei, T3). Resulting cheeses were pickled into its own whey. The low-salted cheese was pickled for 6 months and the high-salted cheese after 9 months. Moisture, salt and yield of low and high-salt cheeses decreased with adding starter culture, while an increase was noticed in the acidity, soluble nitrogen (SN) and total volatile fatty acids (TVFA). High salt cheeses had significantly higher values for moisture, salt and yield with significantly lower acidity, SN and TVFA than low salt cheeses. The values of moisture, salt and yield gradually decreased during pickling while acidity, SN and TVFA significantly increased. Domiati cheese with starter culture had higher total bacterial count (TBC) than that of control being highest in T3. Increasing the salt in cheese milk resulted in lower TBC. Values of TBC increased in all samples during early pickling and then sharply decreased till the end. There was a remarkable inhibition in the growth of sporeforming bacteria and yeasts & moulds with adding starter culture. Sensory quality attributes of cheese improved with adding starter culture. Pickling of cheese up to different periods led to better flavour as well as body & texture but extending the period beyond caused lower quality. The rate of improvement was faster in cheese of low salt than that from 10% salted milk. Starter culture of Bio Profit (T2) produced cheese with typical ripened cheese flavour and texture and can be recommended for low or high-salt cheeses.