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Recent findings on the production of quantum dots from various carbon sources shed light on their advantages such as sustainability, low toxicity and cost, and one-step synthesis over their heavy-metal counterpart. This paper focused on developing and analyzing the production of carbon quantum dots from glycerol via hydrothermal carbonization and conjugated with Tetraethylenepentamine (TEPA). A 23 full factorial experimental design was applied considering factors: the compositional ratio of TEPA (A), time of exposure (B), and temperature of reaction (C). Statistical analyses revealed experimental factors A and B; and interactions of AB and AC had statistically significant effects on the response variable, quantum yield (QY). Factor C as the main effect was not significant but was included in the statistical model to maintain hierarchy and integrity. Coded and actual statistical models were presented here.