Quantitative trait locus (QTL) main effects and QTL by environment (QTL × E) interactions for seven agronomic traits (grain yield, days to heading, days to maturity, plant height, lodging severity, kernel weight, and test weight) were investigated in a two-row barley (Hordeura vulgare L.) cross, Harrington/TR306. A 127-point base map was constructed from markers (mostly RFLP) scored in 146 random double-haploid (DH) lines from the Harrington/TR306 cross. Field experiments involving the two parents and 145 random DH lines were grown in 1992 and/or 1993 at 17 locations in North America. Analysis of QTL was based on simple and composite interval mapping. Primary QTL were declared at positions where both methods gave evidence for QTL. The number of primary QTL ranged from three to six per trait, collectively explaining 34 to 52% of the genetic variance. None of these primary QTL showed major effects, but many showed effects that were consistent across environments. The addition of secondary QTL gave models that explained 39 to 80% of the genetic variance. The QTL were dispersed throughout the barley genome and some were detected in regions where QTL have been found in previous studies. Eight chromosome regions contained pleiotropic loci and/or linked clusters of loci that affected multiple traits. One region on chromosome 7 affected all traits except days to heading. This study was an intensive effort to evaluate QTL in a narrow-base population grown in a large set of environments. The results reveal the types and distributions of QTL effects manipulated by plant breeders and provide opportunities for future testing of marker-assisted selection. M OLECULAR MAPS of plant genomes, used in conjunction with phenotypic measurements, can provide information about chromosome regions that affect quantitative traits. Although knowing whether such regions represent individual quantitative trait loci (QTL)
Wild oat causes more crop yield losses and accounts for more herbicide expenditures than any other weed species on the Canadian Prairies. A study was conducted from 2001 to 2005 at four Canadian Prairie locations to determine the influence of repeated cultural and herbicidal management practices on wild oat population density, biomass, and seed production, and on barley biomass and seed yield. Short or tall cultivars of barley were combined with normal or double barley seeding rates in continuous barley or a barley–canola–barley–field-pea rotation under three herbicide rate regimes. The same herbicide rate regime was applied to the same plots in all crops each year. In barley, cultivar type and seeding rate were also repeated on the same plots year after year. Optimal cultural practices (tall cultivars, double seeding rates, and crop rotation) reduced wild oat emergence, biomass, and seed production, and increased barley biomass and seed yield, especially at low herbicide rates. Wild oat seed production at the quarter herbicide rate was reduced by 91, 95, and 97% in 2001, 2003, and 2005, respectively, when tall barley cultivars at double seeding rates were rotated with canola and field pea (high management) compared to short barley cultivars at normal seeding rates continuously planted to barley (low management). Combinations of favorable cultural practices interacted synergistically to reduce wild oat emergence, biomass and seed production, and to increase barley yield. For example, at the quarter herbicide rate, wild oat biomass was reduced 2- to 3-, 6- to 7-, or 19-fold when optimal single, double, or triple treatments were combined, respectively. Barley yield reductions in the low-management scenario were somewhat compensated for by full herbicide rates. However, high management at low herbicide rates often produced more barley than low management in higher herbicide rate regimes.
As a weed, wheat has recently gained greater profile. Determining wheat persistence in cropping systems will facilitate the development of effective volunteer wheat management strategies. In October of 2000, glyphosate-resistant (GR) spring wheat seeds were scattered on plots at eight western Canada sites. From 2001 to 2003, the plots were seeded to a canola–barley–field-pea rotation or a fallow–barley–fallow rotation, with five seeding systems involving seeding dates and soil disturbance levels, and monitored for wheat plant density. Herbicides and tillage (in fallow systems) were used to ensure that no wheat plants produced seed. Seeding systems with greater levels of soil disturbance usually had greater wheat densities. Volunteer wheat densities at 2 (2002) and 3 (2003) yr after seed dispersal were close to zero but still detectable at most locations. At the end of 2003, viable wheat seeds were not detected in the soil seed bank at any location. The majority of wheat seedlings were recruited in the year following seed dispersal (2001) at the in-crop, prespray (PRES) interval. At the PRES interval in 2001, across all locations and treatments, wheat density averaged 2.6 plants m−2. At the preplanting interval (PREP), overall wheat density averaged only 0.2 plants m−2. By restricting density data to include only continuous cropping, low-disturbance direct-seeding (LDS) systems, the latter mean dropped below 0.1 plants m−2. Only at one site were preplanting GR wheat densities sufficient (4.2 plants m−2) to justify a preseeding herbicide treatment in addition to glyphosate in LDS systems. Overall volunteer wheat recruitment at all spring and summer intervals in the continuous cropping rotation in 2001 was 1.7% (3.3 plants m−2). Despite the fact that volunteer wheat has become more common in the central and northern Great Plains, there is little evidence from this study to suggest that its persistence will be a major agronomic problem.
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