Eight wild plant species namely Tragia involucrata L., Cleistanthus collinus (Roxb.)Benth. Ex Hook.f., Sphaeranthus indicus L., Vicoa indica (L.) Dc., Allmania nodiflora (L.) R.Br. ex wight., Habenaria elliptica Wight., Eriocaulon thwaitesii Koern. and Evolvulus alsinoides L. were used for phytochemical extraction with four different solvents. Antibacterial activity of these plants was studied against Escherichia coli NCIM 2065 using Kirby Bauer agar disc diffusion assay. Effective antibacterial activity was shown by T. involucrata acetone extract (27.3 mm), compared to standard medicinal drug amoxicillin (28.3 mm). Minimum inhibitory concentration (MIC) of T. involucrata extract was 15 mg/mL and hence, it could be pursued further for obtaining phytomedicine. Biochemical constituents of T. involucrata fresh leaf were: sugars (55 mg/g), starch (0.7182 mg/g), proteins (0.0166 mg/g) and lipids (170 mg/g). Alkaloids, tannins, phenolic compounds, flavonoids and steroids were also observed qualitatively.
Common carp, Cyprinus carpio (40 ± 10 g) were inoculated through intramuscular route (2.5 · 10 5 cfu ml )1 ) with the fungal pathogen, Aphanomyces invadans. In the infected group, the total erythrocyte count (RBC), total leucocyte count (WBC), haematocrit (Hct), haemoglobin (Hb), lymphocyte (LYM), monocyte (MON), neutrophil (NEU) and eosinophil (EOS) deviated significantly (P < 0.05) from control values on days 24 and 36. Beginning on day 12 of infection, the fishes were dip treated for 24 days for 5 min daily in water incorporated with 1% aqueous leaf extract of Azadirachta indica (neem); the induced lesion gradually healed and the haematological values attained were near normal (P > 0.05) when compared with the control group.
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Moringa oleifera is a native on Indian sub-continent and also known for its medicinal property for a long time in Indian history. However in the recent past more and more scientists were interested in identifying the anti-oxidant compound from the plant. The study aimed at identifying effective solvent for anti-cancer activity from M. oleifera. In the present study leaf, pod and bark samples were collected and air dried and their crude extract were isolated using ethanol, methanol petroleum ether, ethyl acetate and water to study their isolation capacity using different methods for alkaloids saponins, protein, flavonoids, carbohydrates, tannins, terpinoids, phenols, glycosides and phytosteroids showed presence in all the components of leaf, pod and bark in one or the other tests however, the extraction was better in petroleum ether, acetone, ethanol and methanol. GCMS analysis of compounds present in the crude extract using acetone, methanol and ethanol revealed six major compounds which were commonly isolated and most of them had anti-oxidant capacity.MTT assay of crude extract showed effective cell inhibition capacity for Hela cell and MCF7 cell lines. Methanol solvent was found to be effective for Hella cell lines and Ethanol was found to be effective for MCF cell lines.
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