Background: Seroprevalence of IgG antibodies against SARS-CoV-2 is an important tool to estimate the true extent of infection in a population. However, seroprevalence studies have been scarce in South East Asia including India, which, as of now, carries the third largest burden of confirmed cases in the world. The present study aimed to estimate the seroprevalence of the anti-SARS-CoV-2 IgG antibody among hospitalized patients at one of the largest government hospital in India. Method: This cross-sectional study, conducted at a tertiary care hospital in North India, recruited consecutive patients who were negative for SARS-CoV-2 by RT-PCR or CB-NAAT. Anti-SARS-CoV-2 IgG antibody levels targeting recombinant spike receptor-binding domain (RBD) protein of SARS CoV-2 were estimated in serum samples by the ELISA method. Results: A total of 212 hospitalized patients were recruited in the study with mean age (+/-SD) of 41.2 (+/-15.4) years and 55% male population. Positive serology against SARS CoV-2 was detected in 19.8% patients(95% CI 14.7-25.8). Residency in Delhi conferred a higher frequency of seropositivity 26.5% (95% CI 19.3-34.7) as compared to that of other states 8% (95% CI 3.0-16.4) with p-value 0.001. No particular age groups or socio-economic strata showed a higher proportion of seropositivity. Conclusion: Around, one-fifth of hospitalized patients, who were not diagnosed with COVID-19 before, demonstrated seropositivity against SARS-CoV-2. While there was no significant difference in the different age groups and socio-economic classes; residence in Delhi was associated with increased risk (relative risk of 3.62, 95% CI 1.59-8.21) Key Words: SARS-CoV-2 IgG Antibody, Seroprevalence, Hospitalized patient, COVID-19

BACKGROUND Thyroid hormones play an important role in the regulation and production of red blood cells. Thyroid dysfunction induces different effects on blood cells such as anaemia, erythrocytosis, leucopenia, thrombocytopenia and alteration in red cell indices. In this study, we wanted to compare the changes in haematological parameters of thyroid dysfunction patients with those of euthyroid group. METHODS This was a retrospective study done on 310 individuals by collecting data from the medical records. Later the patients were categorized into hypothyroid (33) thyroid stimulating hormone (TSH > 5.5 μIU/mL), hyperthyroid (19) (TSH < 0.3 μIU/mL) and euthyroid (258) (TSH = 0.3 - 5.5 μIU/ml) groups. The haematological parameters of all these patients were obtained by 5-part automated cell count analyser. Finally, the obtained data was analyzed by statistical package for social sciences (SPSS) software. RESULTS The data obtained from the analysis revealed statistically significant (P < 0.05) difference between hypothyroidism, hyperthyroidism and euthyroidism in mean red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW), white blood cell (WBC) count and platelet count but the difference was not significant for mean haemoglobin, mean corpuscular haemoglobin concentration (MCHC) (P > 0.05). The mean haemoglobin was lower in hypothyroid patients when compared to euthyroid and hyperthyroid patients. The RBC count (P < 0.007), MCH (P = 0.002) and RDW (P < 0.001) showed statistically significant difference between hypothyroidism and euthyroidism, MCV (P = 0.005) showed statistically significant difference between hyperthyroid and euthyroid groups. CONCLUSIONS In case of patients with abnormal haematological parameters, thyroid hormones evaluation is necessary. KEYWORDS Hypothyroidism, Hyperthyroidism, Haemoglobin, Blood Count, Red Cell Indices

Background: Organophosphate (OP) poisoning is one of the most common pesticide poisoning in India in adolescents because of its easy availability. Serum pseudocholinesterase levels are commonly used to assess the severity and to know the prognosis in OP compound poisoning. Serum creatine phosphokinase (CPK) levels is another lab parameter which gets deranged in OP poisoning and has been tried in adults to assess the severity and to know the prognosis. Authors objective was to study the correlation of serum pseudocholinesterase and serum CPK in organophosphate poisoning at admission and to compare outcome with serum CPK levels.Methods: All the children in the age group of 1 month to 18 yrs, who were admitted with the history of suspected OP compound poisoning were enrolled for the study. Estimation of cholinesterase and CPK levels were done at admission and after 1 week. Patients were categorised in to latent, mild, moderate and severe cases based on the S. Cholinesterase levels. These values were analysed to see the correlation.Results: Among 34 OP poisoning cases,13(38%) were males and 21(62%) were females. Mean age of study population was12.6+4.25 yrs. The median CPK values in latent, mild, moderate and severe cases were 121.5 IU/L,276.5 IU/L, 308IU/L and 467IU/L respectively (p=0.015). Spearman’s rho Correlation coefficient was -0.522 between S. Cholinesterase and S CPK at admission which was significant. The median serum CPK level after 1week in non survivors was 2498.0IU/L and in survivors was 201.0IU/L (p0.014).Conclusions: There was a strong negative relationship between serum cholinesterase and serum CPK at admission in OP poisoning. Follow up values at 1 week showed that significantly high serum CPK and low cholinesterase, which was also significant and was associated with mortality.