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Bean root rot (a complex of Apharwmyces spp., Fusarium solani f. sp. phaseoli, Pythium spp. and Rhizoctonia solani) affects winter-grown fresh beans at Gympie, Queensland. In 5 trials conducted in 1986-88, cultural practices (depth of planting, depth of cultivation, hilling) and a fungicide treatment were examined as methods of control of the disease. The experiments were sited on a clay-loam soil, and under these conditions, shallow planting (25 mm) was the most effective treatment, significantly (P<0.05) reducing the severity of root rot relative to the standard depth of 50 mm.

Selected plant species and environmental conditions were investigated for their influences on expression of ice nucleation activity by 15 Pseudomonas syringae strains grown on plants in constant-temperature growth chamber studies. Ice nucleation frequencies (INFs), the fraction of cells that expressed ice nucleation at-5 or-9°C, of individual strains varied greatly, both on plants and in culture. This suggests that the probability of frost injury, which is proportional to the number of ice nuclei on leaf surfaces, is strongly determined by the particular bacterial strains that are present on a leaf surface. The INFs of strains were generally higher when they were grown on plants than when they were grown in culture. In addition, INFs in culture did not correlate closely with INFs on plants, suggesting that frost injury prediction should be based on INF measurements of cells grown on plants rather than in culture. The relative INFs of individual strains varied with plant host and environment. However, none of seven plant species tested optimized the INFs of all 15 strains. Similarly, incubation for 48 h at near 100% relative humidity with short photoperiods did not always decrease the INF when compared with a 72 h, 40% relative humidity, long-photoperiod incubation. Pathogenic strains on susceptible hosts were not associated with higher or lower INFs relative to their INFs on nonsusceptible plant species. The ice nucleation activity of individual bacterial strains on plants therefore appears to be controlled by complex and interacting factors such as strain genotype, environment, and host plant species.