BackgroundAgouti and Extension loci control the relative amount of eumelanin and pheomelanin production in melanocytes that, in turn, affects pigmentation of skin and hair. The Extension locus encodes the melanocortin 1 receptor (MC1R) whose permanent activation, caused by functional mutations, results in black coat colour, whereas other inactivating mutations cause red coat colour in different mammals.ResultsThe whole coding region of the MC1R gene was sequenced in goats of six different breeds showing different coat colours (Girgentana, white cream with usually small red spots in the face; Maltese, white with black cheeks and ears; Derivata di Siria, solid red; Murciano-Granadina, solid black or solid brown; Camosciata delle Alpi, brown with black stripes; Saanen, white; F1 goats and the parental animals). Five single nucleotide polymorphisms (SNPs) were identified: one nonsense mutation (p.Q225X), three missense mutations (p.A81V, p.F250V, and p.C267W), and one silent mutation. The stop codon at position 225 should cause the production of a shorter MC1R protein whose functionality may be altered. These SNPs were investigated in a larger sample of animals belonging to the six breeds. The Girgentana breed was almost fixed for the p.225X allele. However, there was not complete association between the presence of red spots in the face and the presence of this allele in homozygous condition. The same allele was identified in the Derivata di Siria breed. However, its frequency was only 33%, despite the fact that these animals are completely red. The p.267W allele was present in all Murciano-Granadina black goats, whereas it was never identified in the brown ones. Moreover, the same substitution was present in almost all Maltese goats providing evidence of association between this mutation and black coat colour.ConclusionAccording to the results obtained in the investigated goat breeds, MC1R mutations may determine eumelanic and pheomelanic phenotypes. However, they are probably not the only factors. In particular, the surprising not complete association of the nonsense mutation (p.Q225X) with red coat colour raises a few hypotheses on the determination of pheomelanic phenotypes in goats that should be further investigated.

The objectives of this study were 1) to estimate the heritability of lamb survival and growth in the Scottish Blackface breed; 2) to examine the relationship between lamb survival and live BW; and 3) to investigate the possibility of using lamb survival in a breeding program for this breed. The data used for the analyses contained information about survival and live BW at different ages on 4,459 animals. The records were collected from 1988 to 2003 in a Scottish Blackface flock. Live BW was recorded every 4 wk from birth to 24 wk. Survival was defined either by perinatal or postnatal mortality (up to weaning at 12 wk), or as cumulative survival to 1, 4, 8, and 12 wk. The pedigree file comprised 1,416 dams and 178 sires. A sire model was used to estimate genetic parameters for binary survival traits. Heritabilities of BW traits, and phenotypic and genetic correlations between BW and between survival and BW were estimated by fitting an animal model. Further, correlations of survival with live BW were estimated by using a Markov chain Monte Carlo threshold model, implemented by Gibbs sampling. The heritability estimates for cumulative lamb survival declined from birth onward (from 0.33 to 0.08), and postnatal survival had a heritability of 0.01. The direct and maternal heritabilities for BW traits ranged from 0.08 to 0.26 and from 0.06 to 0.21, respectively, whereas the maternal environmental component was between 0.04 and 0.16. The genetic correlations between BW traits at different ages were high. The genetic and phenotypic correlations between survival and BW were always positive (ranging from 0.04 to 0.54), so there was no antagonism between these traits. Therefore, it is possible to simultaneously improve both survival and live BW in a breeding program for this breed.

RIASSUNTO -Effetto delloKEY WORDS: heat tolerance, temperature-humidity index (THI), dairy sheep.INTRODUCTION -European Mediterranean countries are characterized by exposure to considerable heat between three and six months annually. High ambient temperature, solar radiation, wind speed and relative humidity, cause the effective temperature of the environment to be above the thermo-neutral zone of the animals (5 to 25ºC; McDowell, 1972) and therefore heat stress occurs (Bianca, 1962). Heat stress is one of the limiting factors in dairy production in hot climates (Johnson et al., 1962) and is hard to account for by management in the extensive grazing-based farming system of Mediterranean dairy sheep where animals are rarely kept indoors. The interest of our study was to investigate if in the Mediterranean area heat stress has an effect on dairy sheep performance. Some studies (Ames et al., 1971;Sevi et al., 2001) on sheep heat stress investigated changes in rectal temperatures, respiration rates, volumes of air inhaled and other physiological functions. Unfortunately, such measurements are costly and not feasible on a large scale in practical farming circumstances, which leads to insufficient data quantity, especially for genetic studies. In the present paper the methodology of Ravagnolo et al. (2000) based on using weather station data for Holstein cattle was applied to Valle del Belice dairy sheep. The animals were investigated with the following aims: to establish the relationship between production and weather conditions using information from a weather station, and to estimate the additive genetic variances of milk production traits and heat tolerance, and therefore to investigate the possibility of future selection for increased heat tolerance.

European Mediterranean countries are characterized by exposure to considerable heat between three and six months annually. High ambient temperature, solar radiation, wind speed and relative humidity, cause the effective temperature of the environment to be above the thermo-neutral zone of the animals (5 to 25ºC; McDowell, 1972) and therefore heat stress occurs (Bianca, 1962)

No abstract