Purpose -The purpose of this paper is to determine the total phenol content (TP) and total flavonoid content (TF) fluctuations in different infusions of lemon verbena. Dried leaves were harvested in May, July and October and prepared at various soaking times (5-20 min). Design/methodology/approach -The Folin-Ciocalteu method was used to determine TP, while TF was determined by the aluminum chloride method. Findings -According to the results, the July harvest had the highest content of TP and TF, followed by May and October. Temperature and soaking time play a role in the proportion of released phenols and flavonoids (p Ͻ 0.05), whereas the content in continuous infusion is higher than infusion (without boiling) .TP and TF in infusion increase until 20 min, while concentration is convergent in continuous infusion after10 minutes, where no significant difference is seen. In the flowering stage, TP is close in comparison with the vegetative stage. Originality/value -Harvest time plays a role in the content, so it is best to prepare infusion from lemon verbena before the flowering period, when people drink it, as the results show. According to the authors' knowledge, this is the first work that studied the variation in phenol and flavonoid content during different stages of growth with soaking time in drenched lemon verbena leaves.
Trial was conducted in greenhouse of the Department of biology / College of Science/University of Diyala during autumn season 2016. The study included two levels of water stress ( S1 /Irrigation for 14 minutes and S2 / Irrigation for 7 minutes/7 ml of water per minute ), three foliar application levels of Salicylic acid SA (0, 75, 150) mg. L−1 and three foliar application levels of Glutamine acid G (0, 1000, 2000) mg. L−1. Some growth indicators viz., (Plant height, Stem diameter, Relative growth rate and Plant Yield were registered. The experiment was designed as a factorial experiment using an RCBD design with three replicates. Water stress decreased the means of all studied indicators, Plant height record 232.44 cm, Stem diameter 8.28mm, Relative growth rate 0.019 g.day−1 and yield 1358 gm plant−1 at the first stress level (Irrigation for 14 minutes / S1 ) while the means of the indicators decreased to183.82 cm, 7.41 mm, 0.016 g day−1 and 972 g plant−1, respectively at the second stress level (Irrigation for 7 minutes / S2 ). Salicylic acid and Glutamine acid increased all the studied indicators. the third level of salicylic and Glutamine spraying (150 mg. L−1, 2000 mg. L−1 ) has exceeded the second level (75 mg. L−1, 1000 mg. L−1 ). The role of Salicylic acid and Glutamine acid was positive to decrease the passive effect of water stress, Plant height record 236.17 cm, Stem diameter 8.57mm, Relative growth rate 0.021 g.day−1 and yield 1893 gm plant−1(the percentage of increase was 37.57%, 40.95%, 50.00%, 179.62%, respectively), also the triple interaction between the second stress level(S2 ) and the spraying at high levels the Salicylic acid and Glutamine SA3 G3 was positive to decrease the effect of water deficit stress, 262.00 cm, 9.22 mm, 0.024g.day−1 and yield 2317gm plant−1 (percentages of increase were 75.05%,58.97%,100.00%and286.17%, respectively).
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