These results designate that N-(Benzimidazol-1-ylmethyl)-4-chlorobenzamide analogues, substituted with halogen functionality, could be used as potential lead for designing more potent anti-inflammatory and antimicrobial agents.

-(2-chloro-phenyl)-benzimidazol-1-ylmethyl]-benzamide, 3q N-[2-(4-chloro-phenyl)-benzimidazol-1ylmethyl]-benzamide and 3r N-[2-(2-bromo-phenyl)-benzimidazol-1-ylmethyl]-benzamidewere found to be most effective antimicrobial compounds. Clotrimazole and ciprofloxacin were used as reference antimicrobial agents. Further, in silico studies were carried out to define the interaction of the title compounds with microbial protein.

Mannich bases are the end products of mannich reaction and are known as beta amino ketone carrying compounds. Mannich reaction is a carbon carbon bond forming nucleophilic addition reaction which helps in synthesizing N-methyl derivatives and many other drug molecules. Mannich base derivatives of benzimidazoles possess many pharmacological properties such as anti-oxidant, anti-inflammatory, anticancer, antiviral, anthelmintic and play an important role in medical field. As these drugs are clinically useful in treatment of microbial infections and exhibit other therapeutic activities also, so this encouraged the development of more potent, novel and clinically significant compounds. In this review synthesis and various biological activities of new mannich bases of benzimidazole derivatives reported is discussed.

Synthesis and Antifungal Activity of 2-Hydroxy-1-naphthalaniline and Its N-Phenyl Derivatives.-In order to study the influence of the hydroxy group in the naphthyl ring on the condensation reaction and their biological activity title compounds such as (I) are synthesized. -(MANRAO, M. R.; SETHI, R. K.; SHARMA, R. C.; KALSI, P. S.; J.

In the present study novel derivatives of 2-substituted benzimidazoles were prepared via Mannich reaction and evaluated for their in vitro antimicrobial activity against two gram negative strains (Escherichia coli and Pseudomonas aeruginosa), two gram positive strains (Bacillus subtilis and Staphylococcus aureus) and fungal strains (Candida albicans and Aspergillus niger).The synthesized compounds were also screened for antioxidant activity.The newly synthesized compounds were characterized by spectral and analytical techniques.The results revealed that all the synthesized compounds have a significant antioxidant and biological activity against the tested microorganisms.