In this present study, biosynthesis of AgNp’s from methanolic extracts of H. Colorata and its wound healing activity was documented. The synthesis of AgNp’s was done by treating AgNO3 solution with an aqueous extract of H. colorata. The production of AgNp’s was confirmed by a color change of the solution from clear to brown color. The reduced AgNp’s were characterized by Scanning Electron Microscope (SEM), UV–vis spectroscopy. From UV analysis peak was observed at 415nm and spherical shaped AgNp’s were observed. The antibacterial activity and Minimum inhibitory Concentration (MIC) of the silver nanoparticles were determined. The results suggest that biosynthesized AgNp’s from aqueous extracts ofH.coloratashowed a significant antibacterial activity against wound pathogens.

The rapid emergence of drug resistance among pathogenic bacteria, especially multidrug-Resistant bacteria, underlines the need to search for new antibiotics. In the present study, actinomycetes were isolated from lichens collected from coconut trees. The actinomycetes were investigated for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Klebsiella pneumonia in order to identify the potential antibiotic producers.MIC was determined and the organism was identified using 16s rRNA sequencing. Crude action extracts showed 11mm and 9mm against MRSA and Klebsiella pneumonia. The isolated actinomycetes were found to be Streptomyces hygro scopicus subspecies. GC-MS analysis identified several novel compounds. Thus, the isolated actinomycetes can be used for the development of new antibiotics for the treatment of MDR infection.

Urinary catheters are regularly used withinside the elderly for each short- or long-term. The biofilm formation occurs on the catheters and leads to urinary tract infection. The present study focus on the development of Sphagneticolatrilobata coated catheters for the prevention of urinary tract infections (UTI). Bioactive compounds were extracted using methanol and MIC was determined. Biofilm inhibition assay was determined by Fluorescent microscopic analysis. 7.5mg/ml extracts showed 13mm against E. coli and 14mm against S. aureus. The extract-coated catheter showed a significant reduction in biofilms from the fluorescent microscopic analysis. Hence Sphagneticolatrilobataflower extract coated catheters can be used to prevent biofilm colonization and urinary tract infections.

Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulates and facilitates plant growth. The present work deals with the isolation, characterization, and identification of indole acetic acid-producing bacteria from the rhizospheric soil. Out of four organisms isolated from serial dilution, Sample-2 confirmed thick bands of IAA production by TLC analysis. 16s rRNA analysis showed the isolated organism was found to be Pseudomonas fluorescence with a similarity of 88%. The isolated organism treat plants showed increased shoot and root length in plant growth analysis. In conclusion, the isolated IAA-producing bacteria can be used as efficient biofertilizer inoculants to promote plant growth.