Lactobacilli are GRAS organisms and are important members of gut microbiota. They have been strongly recommended as probiotics because of many benefits provided by them to overall human health. Human gut lactobacilli with salutary properties can provide additional advantages. Limosilactobacillus fermentumL1 MW600457, Limosilactobacillus fermentumL3 MW600480, Limosilactobacillus fermentumL4 MW600464, Limosilactobacillus fermentumL5 MW600493, Limosilactobacillus fermentumL6 MW600495, Limosilactobacillus fermentumL7 MW600496, Limosilactobacillus fermentumL8 MW485761, Lactiplantibacillus plantarumL9 MW485746 and Ligilactobacillus salivariusL10 MW600498 with in vitro probiotic properties were explored for salutogenic characteristics. Salutary properties like β-galactosidase activity, anthelminthic property assay, anti-inflammatory assay, anti-diabetic study, cholesterol assimilation assay and biofilm assay were performed. All the isolates were positive for β-galactosidase activity. The anthelminthic property with minimum paralysis time and death time between 16-25 min was shown by Limosilactobacillus fermentumL8 MW485761. Anti-inflammatory activity with bovine serum albumin was maximum at 200 μg ml−1 concentration. α—amylase inhibitory activity was maximum for Limosilactobacillus fermentumL8 MW485761 (51%). Maximum cholesterol assimilation was reported for Limosilactobacillus fermentumL6 MW600495 (76%). Strong biofilm forming ability for all isolates improved the colonization and stability as probiotics. Human gut lactobacilli with salutary properties can make an efficient probiotic.