Can flow cytometric detection of platelet activation early in pregnancy predict the occurence of preeclampsia ? A prospective study Konijnenberg, A.; van der Post, J.A.M.; Mol, B.W.J.; Schaap, M.C.L.; Lazarov, R.; Bleker, O.P.; Boer, K.; Sturk, A.
Published in:American journal of obstetrics and gynecology
DOI:10.1016/S0002-9378(97) Link to publication Citation for published version (APA): Konijnenberg, A., van der Post, J. A. M., Mol, B. W. J., Schaap, M. C. L., Lazarov, R., Bleker, O. P., ... Sturk, A. (1997). Can flow cytometric detection of platelet activation early in pregnancy predict the occurence of preeclampsia ? A prospective study. American journal of obstetrics and gynecology, 177, 434-442. https://doi.org/10.1016/S0002-9378(97)70212-6
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Amsterdam and Leiden, The NetherlandsOBJECTIVES: An increased platelet activation status is present in patients with preeclampsia. Our purpose was (1) to establish by means of flow cytometry whether platelets circulate in an activated state during the first and second trimesters of pregnancy and (2) to establish whether early platelet activation predicts the onset of preeclampsia. STUDY DESI6N: Consecutively, 244 pregnant women were included in a prospective study design. Platelets in whole blood samples from the pregnant women in the first trimester, the second trimester, and after delivery were labeled with the following antibodies associated with platetet activation: anti, CD62P (P-selectin, e-granule secretion), anti-CD63 (GP53, lysosomal secretion), anti-CD31 (GPIla', platelet endothelial cell adhesion molecule-I). The surface antigen exposure was determined by doublelabel flow cytometry with anti-CD42b (GPIb, a platelet-specific monoclonal glycoprotein) to select platelets and platelet-derived materials. Preeclampsia was defined as a diastolic blood pressure ->90 mm Hg and proteinuria ->0.3 gm in a 24-hour urine sample (International Society for Study of Hypertension in Pregnancy criteria). RESULTS: Seventeen of 244 patients had preeclampsia (6.9%). Only first-trimester CD63 expression had an area under the curve >0.5 by receiver-operator characteristic curve analysis and was selected as a possible predictor of preeclampsia. We found a sensitivity of 47% and a spec...
