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Blastocystis sp is the most common intestinal parasite found in humans. Its role in human health is widely discussed due to the high proportion of asymptomatic carriers. Hence the interest of our study to objectify the number of subjects infested by this parasite in our population. This is a retrospective study carried out over a period of ten years, from January 2009 until December 2018, in the parasitology-mycology laboratory of the Avicenna military hospital in Marrakech. 13,255 subjects were included with at least one parasitological examination of the stool, of which 2,799 examinations were positive, with a simple parasite index of 21.11%. Blastocystis hominis was found in 675 subjects with a specific parasite index of 5.09%, and it was co-associated in 446 subjects with a specific parasite index (IPSp) of 3.36%. The most frequent association was that of Blastocystis hominis and E. histolytica/dispar with a rate of (25.5%) of all polyparasitic patients followed by the combination of Blastocystis hominis and Entamoeba coli (20.8%), the combination of Blastocystis hominis and Endolimax nana with a rate of (16, 1%) and lastly Blastocystis hominis and Entamoeba hartmani (5.3% In patients with polyparasitism, the association of Blastocystis hominis with Entamoeba coli was 20.8%, with Endolimax nana with a rate of 16.1% and finally with Entamoeba hartmani with a prevalence of 5.3%. It is always necessary to insist on strict observance of the rules of prophylaxis against oral-fecal contamination.