Background: Niosomes are the non-ionic surface-active agent primarily based vesicles. The Key obstacle of Asenapine maleate have oral bioavailability (<2%) and extensive first pass effect. Objectives: The objective key of the existing work was to formulate Asenapine maleate loaded niosomes using quality by design and rectify it with some evaluation parameters to increase bioavailability, to lead better therapeutic effect and to minimize the side effects. Methods: Formulation of niosomes was done by three methods organic solvent injection method. Factorial design (3 2 ) experiments are significantly used to screen and to observe the effect of independent variables cholesterol and span 60 (X 1 , X 2 ) on dependent variables particle size and entrapment efficiency (Y 1 , Y 2 ). Results:The results revealed optimized formulation ASP-niosome A2 amongst other formulations which was found lowest particle size 84 ± 5 nm and highest % EE 70 ± 2.0%. In-vitro drug release of optimized noisome was found 68 ± 1.20 % at the end of 8 hr and zeta potential was -17.53mV which stabilized the niosomal suspension. Characterization by SEM not only indicated the spherical shape of the niosomes but also confirmed the formation of vesicle. Locomotor activity was found to be significant in in-vivo pharmacodynamic study. Pharmacokinetic study carried out and it showed C max and t 1/2 of 16.12ng/mL and 37.18 hr which is better than reported parameters for drug. Conclusion: Thus, concluded that Asenapine maleate loaded niosomes having effective anti-psychotic activity with increased bioavailability could be prepared successfully by organic solvent injection method using span 60 and cholesterol.

Gingerol is an analgesic, hypoglycemic, hepatoprotective, immune-stimulant, anti-inflammatory agents. In traditional medicine, gingerol has been proven plenty of pharmacological properties such as bactericidal, microbicidal, potent antioxidant, antifertility, tuberculostatic and anticancer. The key objective of this work was to develop, validate and estimate gingerol by RP-HPLC method. According to the ICH guidelines, a stabilityindicating RP-HPLC method was developed and validated. In this method, reverse phase enable Cosmosil C18 column (250 × 4.6 mm, 5μm) was used in isocratic mode. The mobile phase consisted of acetonitrile: methanol: water (52: 8: 40 v/v) delivered at a flow rate of 1.0 mL min -1 . The elute injection volume was 20 µL that was analyzed by a UV detector at 280 nm. Linearity range of the concentration of gingerol was 20 μg mL -1 -140 μg mL -1 and the correlation coefficient R 2 value was found to be 0.997 ± 0.11. Recovery was found to be in the range of 98.16 -100.07 %. The limit of detection (LOD) of gingerol was found to be 2.23 μg mL -1 and limit of quantitation (LOQ) was 5.02 μg mL -1 . Gingerol was subjected to stress conditions including acidic, alkaline, neutral, oxidation, and dry heat degradation. Gingerol was more sensitive to alkaline, dry heat and oxidative degradation and it is stable at acidic conditions. The method was validated according to ICH guidelines.