The essential oil of oregano (‘origanum oil’; thymol type oil from Origanum vulgare) inhibited completely the mycelial growth of Aspergillus niger and A. flaous at 400 μg/ml, while A. ochraceus was inhibited at 600 μg/ml. At 700 μg/ml, thyme oil inhibited the mycelial growth of A. flavus and A. niger but not that of A. ochraceus. Fungal spore germination was inhibited by 600 μg/ml of origanum oil and (with the exception of A. ochraceus) by 700 μg/ml of thyme oil. Under aerobic conditions, the essential oils of oregano (250 μg/ml) and thyme (350 μg/ml) inhibited to some extent the growth of Staphylococcus aureus and Salmonella typhimurium. Pseudomonas aeruginosa was not affected by either oregano or thyme oil at concentrations up to 500 μg/ml. The origanum oil was very effective against Campylohacter jejuni and Clostridiurn sporogenes and thyme oil was very effective against C. jejuni. The antagonistic effect of the two oils on Staph. aureus and Salm. typhimuriutn was greatly enhanced when those organisms were incubated in atmospheres of low oxygen tensions
Simulation models of parallel, rotary, and side-opening milking parlors were built that could predict milking parlor performance according to herd size, number of milking stalls, labor quality, and cow characteristics. The models were validated by statistically comparing the duration of the simulated milking process with actual data collected at 3 dairy farms during 12 mo. Various scenarios were generated to study parlor performance, and the results indicated that for a parlor with up to 14 milking stalls, a side-opening design provided greater capacity than parallel or rotary parlors. Performance of a side-opening parlor was reduced by enlargement up to 20 milking stalls. For 10 to 40 milking stalls, a rotary design gave better performance than a double parallel design in terms of milking process duration and stall utilization. The presented models can serve as a practical tool in designing new parlors or changing operations of existing ones.
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