A Ca2+-dependent actin filament-capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase-I agarose. The protein caps the fast-lowing end of actin filaments but has no fragmenti~ or severing activity. Using Triton X-IOO-extracted cytoskeletons, capping and severing activities of actin-binding proteins become clearly distinguishable from each other.
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