Cytoarchitecture and distributions of acetylcholinesterase-containing fibers and zinc-containing neuropil are described in the primary visual cortex of a metatherian, the Parma wallaby (Macropus parma). Although some cytoarchitectural features of layer III suggest that a subdivision and a corresponding layer IIIa-c terminology may be employed, neurochemistry is more concisely described using a simple layer I-VI terminology. Horizontal acetylcholinesterase-containing fibers in layer I take on a vertical course as they pass through layer II to form a reticulate pattern in upper layer III. Fibers gather into vertical groups in lower layer III and, markedly, in layer IV. No dominant orientation is seen in layer V, whereas an horizontal course is often seen in layer VI. Zinc-containing neuropil is very dense in layers I, II and upper III, where particles are homogeneously distributed. Particles begin to form strands in lower layer III and form conspicuous vertical clusters in an otherwise almost unstained layer IV. There is a sharp transition to a densely and homogeneously stained layer V, a decrease in staining intensity in layer VI, and a further decrease within layer VI towards the white matter. The distribution of acetylcholinesterase-containing fibers in M. parma, a metatherian representative, adds to the number of species-specific patterns observed in eutherians. The distribution of zinc-containing neuropil in M. parma resembles the consistent pattern seen in eutherian species. Thus, pathways incorporating zinc, as a putative neuromodulator of excitatory amino acid receptor responses, may be organized similarly in the cortex of meta- and eutherians.