The purpose of this study was to evaluate the overexpression of cyclin G in colorectal neoplasia, which may be a more frequent event than cyclin D1 during the cell cycle and thus may have a more enhanced therapeutic potential in treating colorectal cancer. Ninety formalin-fixed, paraffin-embedded human colon and rectal specimens were obtained from the Pathology Department of Norris Cancer Center/University of Southern California. The tissues had been obtained after surgical resection between 1995 and 2001, and had been processed by routine clinical histopathologic methods. Ninety-one percent of colorectal tumors had cyclin G overexpression. These cyclin-positive patients were evenly distributed between men and women, and between tumor locations, that is, 36% rectal tumors and 34% right-sided tumors. Thirty-two percent were well differentiated, and 66% were moderately differentiated. Thirty patients (38%) had stage I disease, 16 (20%) had stage II disease, 25 (32%) had stage III, and seven (9%) had stage IV disease. Eight patients (10%) in this group had recurrent disease during follow-up. There was no correlation between cyclin G overexpression and clinical and pathologic characteristics. Cyclin D1 overexpression was found to be present in only 42% of colorectal adenocarcinomas. There was no correlation between cyclin D1 overexpression and clinical and pathologic characteristics. The present study demonstrates that cyclin G overexpression is a frequent event in colorectal cancer. This frequent event in colorectal carcinogenesis may facilitate new therapeutic approaches acting as a target for gene therapy, possibly directed at downregulating cyclin G in colorectal cancer.

Assuring access to high-quality water for its multiple uses is increasingly difficult and relevant, as climate changes are gradually altering the hydrologic cycle and impacting traditional and well-established techniques of water resource management. This manuscript proposes a methodology to assess the impact of climatic variability in pre-established management rules, using spatially interpolated rain gauged data for two future emission scenarios. With them, water allocation and water quality parameters are simulated for the Piracicaba, Capivari, and Jundiaí watersheds (PCJ watersheds) in São Paulo, Brazil, employing comparisons among scenarios of historical and climate modified hydrological series. Five selected water quality indicators are used to confirm that the introduction of climate variation signals worsens water quality parameters, along with a decrease in the capability to meet water demand. This finding suggests the importance of including climate change impact in similar studies in management plans. The results indicate higher stress levels on the watershed when changes in the hydrological regime are introduced by the future conditions modeled and driven by the regional climate model (RCM). Water availability decreases and water quality deteriorates, indicating that stakeholders must take action to progressively implement stricter control measures to achieve the goals established by the watershed master plan regarding the limits or classification set by the body governing the watershed in question. On average, there was an increase of about four times the amount of river stretches (in kilometres), from 29.6 km to 161.9 km outside the limits of the established framework. The average was taken for all parameters as presented in the discussion.