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The eco-friendly phytomediated synthesis of Ag nanoparticles provides better efficient applications than synthetic methods. Ag nanoparticles are considered by researchers due to its specific properties. The phytochemical analysis of Allium Sativum flower extract provides the phytochemicals exist in it. Due to this, the efficient eco-friendly synthesis of Ag nanoparticles via Allium Sativum flower extract has performed. The X-ray diffraction pattern exposes the crystalline nature with the mean crystallite size at 25 nm. The Fourier Transform Infra-Red spectrum displays the phytochemicals involved in the synthesis. The UV-Visible spectrum illustrates the formation of Ag nanoparticles with SPR band at 436 nm. The particle size analyzer demonstrates the size of formed Ag particles in 20-35 nm. The morphological, elemental and structural analyzes demonstrate the formation of spherical shaped Ag nanoparticles. The formation of Ag nanoparticles was confirmed by the appearance of Ag peak in Energy Dispersive X-ray Diffraction spectrum and mapping study. The antimicrobial activity explains the effective antimicrobial effect on human pathogens. The antioxidant activity reports the efficient scavenging property of synthesized Ag nanoparticles. The anti-inflammatory activity reveals the potential ability of protein denaturation on bovine serum albumin. The anti-diabetic activity exhibits much inhibition on α-Amylase. The anticancer activity reports the effective inhibition of synthesized Ag nanoparticles on human MCF-7 breast cancer cells.

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<p><strong>Objective: </strong>Our present study deals with the anticancer property of silver nano bio conjugates synthesized from the methanolic extract of <em>Piper betle</em> leaves and the pure compound eugenol.</p><p><strong>Methods: </strong>The anticancer activity of the nano bio conjugates synthesized from the extract and pure compound, in comparison with their respective non-conjugated raw material, were studied using cancer cell lines. The anticancer activity was analyzed by cell viability and staining techniques. The cell viability was determined by the MTT assay and the apoptosis was evaluated by the AO/EtBr staining method, which was carried out in both the cancerous (oral carcinoma (KB) cell lines) and non-cancerous cells (primary cultured human buccal cells). The phase of cell death was assessed by the flow cytometry followed by the cytotoxicity assessment.</p><p><strong>Results: </strong>The results revealed that <em>Piper</em> <em>betle</em> leaves as well as their active phenolic compound eugenol exhibited strong anticancer activity in KB oral carcinoma cells. The anticancer effect of the extract was attributable to the component phenolics, eugenol respectively in betel leaves, as the phenolics also exhibited strong cytotoxicity in cancer cells. The anticancer activity increased by a marked magnitude when the extract/compound was administered as nano-bio conjugates. The significant observation was the differential effect evoked by the AgNPs, which was non-toxic to non-cancerous buccal cells while evoking a strong cytotoxicity in the cancer cells.</p><p><strong>Conclusion: </strong>The results proved that the phytoconstituents like phenols in the betel leaves play a major role in the reduction of nano bio conjugates. The anticancer properties of silver nano bio conjugates were higher in the conjugated nanoform than the non-conjugated form.</p>

Phenolic compounds are naturally occurring compounds present ubiquitously in plants. They have potential health benefits and substantiate evidence for their nutraceutical applications. Diarylheptanoids are part of the broad class of plant phenolics with structurally divergent compounds. They have been used in traditional medicines and homemade remedies to treat various ailments, as organoleptic additives in foods, and also for aesthetic purposes. With their potential therapeutic and organoleptic characteristics, diarylhepatanoids can be rightly termed as nutraceuticals. This review summarizes the wide range of pharmacological activities of diarylhepatanoids and nutraceutical formulations, with relevance to human health.