This study compared the efficacy of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid, and their combination on growth performance and intestinal health in broiler chickens challenged with S. Typhimurium. Ross 308 male broilers (n = 420) were randomly assigned to one of the 6 treatments, resulting in 5 replicate pens of 14 chicks per treatment. The treatments included a negative control [(NC), no additive, not challenged]; positive control [(PC), no additive, but challenged with S. Typhimurium at d 3 posthatch], and 4 groups whereby birds were challenged with S. Typhimurium at d 3 posthatch and fed diets supplemented with either probiotic [0.1 g/kg Pediococcus acidilactici (PA)], prebiotic [2 g/kg mannan-oligosaccharides (MOS)], organic acid [0.5 g/kg butyric acid (BA)], or a combination of the 3 additives (MA). The S. Typhimurium challenge decreased feed intake, body weight gain and increased feed conversion ratio and reduced jejunum villus height (VH) and VH to crypt depth (CD) ratio (P < 0.05). Birds on the MA treatment exhibited similar performance to birds on the NC treatment (P > 0.05) and had a lower population of Salmonella in the ceca compared with birds on the PC treatment, at d 14 and 21 post-challenge (P < 0.05). The lowest heterophil to lymphocyte ratio was observed in birds on the MA and NC treatments (P < 0.05). Birds fed diets supplemented with MA or PA had greater VH and VH: CD ratio than birds on the PC treatment at d 7, 14 and 21 d post-challenge (P < 0.05). Suppressed amylase and protease activity was observed as a result of the S. Typhimurium challenge; the enzyme levels were restored in birds fed the additive-supplemented diets, when compared to the birds on the PC treatment, particularly at d 21 post-challenge (P < 0.05). These results indicate that dietary supplementation with a combination of PA, BA, and MOS in broiler chickens could be used as an effective tool for controlling S. Typhimurium and promoting growth performance.

The effect of cinnamon powder (CNP) feeding on some blood metabolites in broiler chicks was studied in a 49-day experiment. The feeding program consisted of a starter diet until day 21, a grower diet until day 42 and a finisher diet until day 49. There were five treatments: a negative control diet, without the additive (control: T1); 250 mg/kg (T2); 500 mg/kg (T3); 1000 mg/kg (T4); and 2000 mg/kg (T5) of CNP in the diet. A total of 320 one-day-old mixed-sex broilers (Ross 308) were distributed into 16 pens with 20 chickens each, comprising 3 replicates per treatment (except for T1, which had 4 replicates). Serum glucose was measured weekly and reported periodically. At the end of the experiment serum cholesterol, triglycerides, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined. There were no significant differences among treatments regarding broiler performance and carcass characteristics, except for the finisher phase, when T2 resulted in a significant decrease in feed conversion ratio and weight gain (p<0.05). In the lowest level of cinnamon supplementation (T2), a significant increase in glucose level in starter, finisher and overall rearing period was observed, as well as an increase in triglyceride levels at the finisher phase as compared to T4 and T5 (p<0/05). However, cholesterol, AST and ALT levels were not statistically different among treatments. Therefore, it was concluded that the use of cinnamon powder can affect on some blood metabolites and broiler performance