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A mini bioreactor (3.0 mL volume) has been developed and shown to be a versatile tool for rapidly screening and quantifying the response of organisms on environmental perturbations. The mini bioreactor is essentially a plug flow device transformed into a wellmixed reactor by a recycle flow of the broth. The gas and liquid phases are separated by a silicone membrane. Dynamic mass transfer experiments were performed to determine the mass transfer capacities for oxygen and carbon dioxide. The mass transfer coefficients for oxygen and carbon dioxide were found to be 1.55 AE 0.17 Â 10 À5 m/ s and 4.52 AE 0.60 Â 10 À6 m/s, respectively. Cultivation experiments with the 3.0 mL bioreactor show that (i) it can maintain biomass in the same physiological state as the 4.0 L lab scale bioreactor, (ii) reproducible perturbation experiments such as changing substrate uptake rate can be readily performed and the physiological response monitored quantitatively in terms of the O 2 and CO 2 uptake and production rates. ß

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In this paper we discuss improvements in mechanical, electrical and software design, which we did to become RoboCup 2014 world champion. Regarding hardware and control our progress includes first steps towards improved passing accuracy via velocity feedback control on the shooting lever. In terms of intelligent gameplay we have worked on creating possibilities for in-game optimization of strategic decisions. Via qr-code detection we can pass coaching instructions to our robots and with a basic machine learning algorithm success and failure after free-kicks is taken into account. In the final part of this paper we briefly discuss progress we have made in designing a four-wheeled soccer robot with a suspension system.

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