Controlled mycorrhizal initiation as a means to improve root development in somatic embryo plantlets of hybrid larch {Larix X eurolepis) ¥. Piola, R. Rohr and P. von Aderkas Piola, F., Rohr, R. and von Aderkas, P. 1995. Controlled mycorrhizal initiation as a means to improve root development in somatic embryo plantlets of hybrid larch (Larix x eurolepis). -Physioi. Plant. 95: 575-580.This study represents the first report of in vitro myconhization of somatic embrj'O-derived plantlets. Effects of fungi on the rooting of plantlets as well as on their ultrastruaure were studied. Embryo-de rived plantlets of a hybrid larch (Larix x eurolepis Henry) were grown aseptically in the presence of four ectomycorrhizal ftingi. One montfi after inoculation with Laccaria laccata, Hebeloma cylindrosporum and Pisolithus tinctorius, the number of plantlets which had developed a well-growing root system was significantly improved compared with controls. Furthertnore, root branching was strongly stimulated by L. laccata and Ft. cylindrosporum, which were more efficient species than P. tinctorius and Suiilus grevillei. Development of the root system was concomitantly accompanied by an enhancement of shoot growth, except for plantlets inoculated with S. greviliei. which died after three to six months. At the ultrastructural level, cross-sections of lateral roots made six months after inoculation, showed the presence of a Hardg net in the case of L. laccata and H cylindrosporum, whereas no such net was observed with the two other fungi. With 5. greviliei, some perforating hyphae penetrated the cortical cells and resulted in cell death. Evidence of a relationship between structure and efficiency of the association was found.