Sugar cane bagasse is a waste from the processing of sugar cane in a sugar factory. Bagasse is obtained about 25% of the total weight of sugar cane which is used as raw material. Nutrients contained in bagasse approximately protein 1-4%, lipid<4%, ash 2-8%, crude fiber 20-38% and NFE 52-61% (in dry weight). Lignocellulosic component found in bagasse were lignin (11-27%), cellulose (26-49%) and hemicellulose (16-33%). This research aimed to improve bagasse into a fish feed ingredient. The study was conducted using a completely random design with four treatments and three replications. The treatment is carried out by mixing pre-treated bagasse with crude enzyme extract from Bacillus subtilis [1/1], [1/0.75], [1/0.5], [1/0.25] w/v which performed at optimum conditions, i.e. 50°C, pH 5.5 and incubation time 72 hours. The results showed that the treatment [1/1] w/v gave the best results. The improved bagasse had a protein content of 24.92%, ash 6.83%, crude fibre 11.56%, (in dry weight). Dissolved protein and reducing sugar increased to 417.60 mg/L and 442.63 mg/L, respectively. Lignin decreased to 2.81%. So the improved sugar cane bagasse was feasible to be used as a fish feed ingredient.

Shrimp head is a feedstuff that potential to be developed as an alternative source of protein to substitute fish meal. It is because the shrimp head contains a high protein and its availability in Indonesia is quite abundant. However, this protein can not be utilized by fish because it contains high crude fiber (chitin) and ash which is difficult to digest. This study aimed to improve the quality of shrimp head meal by fermentation using Bacillus sp PAS7 at different inoculum doses and evaluate its nutrient content. The study was conducted in a completely randomized design (CRD) of four treatments and three replications. The treatment was carried out by varying concentrations of 0, 3, 5 and 7% per gram (weight) of shrimp head meal (v / w). Fermentation was done for three days at 28°C. The parameters measured were reducing sugar, soluble protein and proximate analysis (crude protein, crude fiber, ash and crude fat). The results showed that all dosage treatments had crude protein, soluble protein and crude fiber, which were better than control. Where dose of 7% had the highest soluble protein content (6.25 mg mL−1) and the lowest crude fiber content (15.02%) compared to other treatments.