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Scanning electrochemical microscopy (SECM) is a technique of interest to obtain information about chemical, electrochemical, kinetics and topographical activities of materials. Even though this technique is applied to non-biological systems, as in studies of metal corrosion, recently it has been enhanced, being widely used in research labs using cell culture. In general, this technique analyzes and tracks the electric current using ultra/microelectrodes placed near the surface of a sample submerged in electrolyte solution. The action of electrical signals has extensive relationship with the human body, its physiological effects are based on the consequent improvement of nutrition, oxygenation and cells metabolism as a whole, and these signals also stimulate fibroblasts to produce more and better collagen. Therefore, this study was based on in vitro monitoring of electrochemical current in fibroblasts cell culture before and after use of cosmetic formulations containing ingredients for anti-aging treatment. Electrochemical current was evaluated in cell culture of fibroblasts by scanning cells surface and using chronoamperometry technique to measure the electrochemical current, using three different cosmetic formulations treatment: tonic, gel cream and serum, applied separately and also in combined use, in order to verify the change of bioelectricity.