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Conclusion:Influence of ethnicity on fetal nasal bone length was not significant. Our preliminary results showed for the Brazilian population was not necessary the first-trimester screening adjustment for ethnicity. Objective: To assess the relationship between amniotic fluid sample volume, culture time and culture failure in prenatal diagnosis. Method: We retrospectively evaluated the results of 225 cases of genetic amniocentesis performed between 16 and 20 weeks of gestation. Cases were separated into four groups according to the amount of amniotic fluid obtained: Group A (n = 12, 1 to 5 mL); Group B (n = 23, 6 to 10 mL); Group C (n = 10, 11 to 15 mL); Group D, (n = 180, 16-20 mL). Results: Culture time was significantly longer in Group A than in the other groups. (median 19 days vs. 12.5, 10, 11 days). Even if differences in culture time within Groups B to D were not significant, there was a trend towards an increase in culture time with decreasing amniotic fluid volumes. Moreover, risk of culture failure was also increased with smaller volumes. There were no culture failures in Groups B, C and D but two culture failures in Group A. Conclusion: When necessary, small amniotic fluid volumes (at least 10 mL) may be used for fetal karyotyping with confidence after information of the family. Small volumes of amniotic fluid increase culture time and rate of culture failure. P01.33 P01.34 Evaluation of complications after the genetic amniocentesis 2nd Department of Obstetrics and Gynecology, Warsaw, PolandObjective: To evaluate complications after genetic amniocentesis. Methods: Retrospective analysis of 1153 women who underwent genetic amniocentesis from 1999 to 2005, which was done by ultrasound-guided insertion of a 22-gauge needle. The mean maternal age was 37 ± 4 years. 1148 (99%) women were in singleton pregnancy, 4 (0.03%) in twin pregnancy and one (0.08%) in triplet pregnancy. Results: Amniocentesis was performed between 12 and 32 weeks' gestation (mean 14 ± 1 weeks). The most common indication for amniocentesis was maternal age over 35 year -988 (86%). 17 (1.4%) patients had pregnancy loss after amniocentesis: 7 (0.6%) women had missed abortion and 10 (0.8%) had amniotic fluid leakage. 1 (0.1%) had pain in hypogastrium after the procedure, but she delivered at term. In 8 (0.7%) cases the cell culture of amniotic fluid was unsuccessful and procedure was repeated. Conclusions:The pregnancy loss rate in women who underwent genetic amniocentesis was 1.4%. P01.35Ultrasound screening for chromosomal abnormalities by fetal nuchal translucency measurement between 11-14 weeks of gestation Federal University of Espirito Santo, UFES, University Hospital HUCAM, Vitoria Medfetus, BrazilObjective: To assess the feasibility of nuchal translucency [NT] measurement as a screening tool for chromosomal anomalies between 11-14 weeks of gestation. Methods: A longitudinal prospective follow up study was carried out at a tertiary referral center including 3263 singleton pregnancies between 11 and 14 weeks of gestation. Two experi...

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