AbstrakLipid terstruktur dengan medium chain fatty acid (MCFA) pada posisi luar dan polyunsaturated fatty acid (PUFA) pada posisi sn-2 memiliki nilai gizi dan absorbsi yang sangat baik. Dalam penelitian ini lipid terstruktur disintesis secara langsung melalui asidolisis enzimatis antara minyak ikan dan asam laurat. Reaksi dikatalisis oleh lipase dedak padi. Tujuan penelitian ini adalah mempelajari perilaku dari reaksi asidolisis enzimatik minyak ikan tuna dan asam laurat, dengan kajian pengaruh biokatalis lipase dedak padi terhadap hasil asidolisis. Target yang ingin dicapai berupa data-data teknis laboratorium untuk perancangan, scale-up dan pengoperasian proses yang meliputi kinetika reaksi, studi produktifitas asam lemak, kondisi operasi yang optimum dan analisa tekno-ekonomi. Hasil penelitian menunjukkan bahwa konsentrasi lipase dan suhu reaksi optimum berturut-turut 10% dan 50 o C. Rasio mol optimum minyak ikan dan asam laurat adalah 1:10, dihasilkan inkorporasi asam laurat mencapai 62,8 mol%. Pada waktu inkubasi 12 jam, trigliserida menurun seiring dengan meningkatnya waktu inkubasi, sedangkan digliserida meningkat seiring dengan meningkatnya waktu inkubasi. Pada suhu reaksi di atas 50 o C, trigliserida menurun seiring dengan meningkatnya suhu reaksi. Metode interesterifikasi ini cukup efektif untuk mensintesis lipid terstruktur spesifik. Lipase dapat digunakan dengan baik untuk sintesa Lipid Terstruktur dari minyak ikan tuna dengan asam laurat. Kondisi optimum reaksi adalah pada suhu 50 o C, konsentrasi lipase 10%, perbandingan ratio substrat (Minyak ikan tuna : asam laurat) 1:10 selama 12 jam. Profil gliserida dari hasil asidolisis enzimatis adalah 78,1 % trigliserida, 32,2 % digliserida dan 11,9% monogliserida Kata kunci: Lipid, lipase, dedak padi, minyak ikan tuna Abstract Lipase Rice Bran Biocatalystator For Asidolysis Process Tuna Oil And Lauric AcidLipid structured with medium chain fatty acids (MCFA) in the outer position and polyunsaturated fatty acid (PUFA) in sn-2 position has excellent nutritional value and absorption. In this study structured lipids were synthesized directly through enzymatic acididisation between fish oil and lauric acid. The reaction was catalyzed by a specific lipase of 1.3 from the tertiary carotid rugose. The aim of this study was to study the behavior of enzymatic acidic reactions of tuna and lauric acid oils, with the study of the effect of rice bran biocatalyst on acidic acid yield. The targets to be achieved are technical laboratory data for design, scale-up and operation of processes including reaction kinetics, fatty acid productivity studies, optimum operating conditions and techno-economic analysis. The results showed that the optimum lipase concentrate and temperature of the reaction were 10% and 50 o C, respectively. The mole ratio of fish oil and lauric acid was 1:10 in which the incorporation of lauric acid was 62,80% (mol). Incubation time, 12 h, triglyceride decreased with an increase in incubation time. In contrast, the diglyceride increased with an increas...
Fresh water snail (Pila ampullacea) is large fresh water snail that can be found in rice fields, ponds, lakes and marshes. Most of them live in paddy fields and become pests for its plant thus the farmer tries to eradicate. Whereas, aquatic animals especially snail contain high level of protein with an amino acid profile similar with the meat land animals, thus making it beneficial to health in countries where snail exists. Thus, fresh water snail can be used as a raw material in producing foods or snacks, one of the famous snack in Southeast Asian countries is cracker. Pila ampullacea was obtained from the swamp and was cleaned it up by removed the flesh from its shell and used the water then production of the crackers. Nutrition contents of the fresh water snail cracker is protein (10.67%) and lipid (0.06%). The organoleptic analysis of crispiness was shown good result with mean score is 4.1.
Ginger is one of the herbal plant species that is used as cook seasoning because it can add a distinctive flavor to foods. Ginger also has so many substance contents that is needed for the human body, such as essential oil (0,5-5,6%), zingiberon, zingeberin, zingibetol, barneol, kamfer, folandren, sineol, gingerin, vitamin (A, B1, and C), carbohydrate (20-60%), resin and organic acid. This research aims to find the value of antioxidant and quality requirements of traditional beverage powder from ginger powder. The tools which are used in the making of ginger powder with the addition of betel leaf extract are: knife, pan, filter, blender, stove, and spatula. While the tools for analyzing are beaker glass, measuring cylinder, test tube, measuring flask, oven, petri dish, Erlenmeyer. The ingredients which are used in the making of ginger powder are : ginger, betel leaf, sugar and water. The result of this research found that water contain of instant ginger betel is 2,080% while ash content of instant ginger betel is 0,533%. This research also analyzes the level of metal contamination, where the metal that is tested is Pb, Cu and Zn metal. The result of the Cu metal contamination is equal to 0,198 ppm, Zn metal is 0,527 ppm, while the Pb metal contamination is undetected. The research is also analyzing the content of antioxidant and found that the content of antioxidant is 41,632%. The conclusion is the content of metal contamination is Cu, Zn and Pb, while water content and ash content is still the same with SNI 01-4320-1996.
AbstrakBeberapa formula insektisida tidak efektif untuk membasmi vektor penyakit Demam berdarah dengue sehingga digunakan formula kombinasi beberapa insektisida golongan organofosfat dan karbamat yang terbukti memenuhi standar Komisi Pestisida Indonesia. Penelitian ini bertujuan untuk menguji efikasi formulasi campuran golongan organofosfat dan karbamat terhadap vektor DBD Aedes aegypti strain Semarang. Formulasi insektisida yang digunakan mempunyai komposisi propoxure 17 %, Malathion 3 %, agrisol 13 %, DBS 7 %, Xyline 55 %, dan additive 5 % (% berat). Uji efikasi (uji LD 50 ) dilakukan dengan menggunakan hewan uji nyamuk Aedes aegypti strain kota Semarang. Sebelum digunakan uji efikasi, formula insektisida cair diencerkan 10 kali dengan pelarut adthiner. Uji efikasi menunjukkan bahwa semua hewan uji tersebut 90% mati pada dosis 0,250 cc/L sehingga disimpulkan bahwa dosis yang terbaik untuk digunakan pada foging adalah 0,250 cc/ L. Abstract Effication Test Of Insecticide Formula With Active Ingredient Of Propoxure And Malathion To DBD Disease VectorSeveral insecticide formulae have not been effective to eridicate the vector of dengue fever disease therefore the combination some insecticide of organophosphate and carbamate has been proved to match with Indonesian Pesticide Commision. The present work was aimed to do the effication test of insecticide formulation of organophosphate and carbamate on vector of DBD. Insecticide formulation were consisted of 17 % propoxure, 3 % Malathion, 13 % agrisol, 7 % DBS, 55 % Xyline, and 5 % additive (% weight). Effication test (LD 50 ) use mosquitoes of Aedes aegypti Semarang strain. Prior the test the insecticide formulation was diluted 10 times with adthiner solvent. Effication test showed that 90% mosquitoes was killed at dose of formulation 0,250 cc/L. Therefore it is conclused that 0,250 cc/L of insecticide formulation was recommed for Aedes aegypti fogging.
Formula yang ada dipasar bebas hanya dapat membunuh nyamuk saja, maka dalam penelitian ini akan dilakukan Produksi formulasi insektisida sebagai insektisida alternative pembunuh nyamuk dan jentik, dimana agrisol dan dodecyl benzen sulfonat merupakan emulsifier nonionic dan anionic sert bersifat hydrophilic. Penelitian ini bertujuan untuk menentukan konsentrasi emusifier yang tepat dalam proses formulasi insektisida, sehingga kestabilan emulsi antara minyak dengan air dalam waktu yang cukup lama Pada percobaan pembuatan emulsifier konsentrat kemudian dilakukan uji kestabilan emulsi antara minyak dan air serta uji effikasi Maka dapat diproduksi formula yang digunakan sebagai bahan dasar insektisida dalam membasmi nyamuk dan jentik nyamuk Aedes aigepty. Disamping itu juga dapat menjadi formula obat nyamuk (insektisida) alternatif yang mudah diaplikasikan oleh masyarakat. Hasil penelitian menunjukan bahwa emulsifier concentrat yang terbaik adalah pada percobaan dengan formula komposisi: 17,38% propoxure; Malathion 17,39% agrisol; 13,01% DBS, 47,83 % Xyline dan 4,35% additive dalam % berat. Dengan total emulsifier yang digunakan 30,5 % w/w equivalent dengan 30 EC Dari uji kestabilan antara minyak dan air didapat pelarut yang terbaik adalah thiner, dengan membentuk micro emulsi pada lapisan air sampai 12 minggu lebih, kestabilan emulsi tercapai pada CMC 0,42 % v/v pada lapisan minyak. Dari uji effikasi dengan pengencer thiner sebanyak 10x setelah dianalisa profitabilitas sesuai LD 50, didapat dosis . 0,025 -0,25 % (v/v), baik untuk telur nyamuk maupun jentik Aedes aegypti dengan prosentasi kemation 45-90% Effectiveness of Solvent Usage as a Solvent for Insecticide Formula in Eradicating Aedes Aegypti Mosquitoes to Prevent Dengue Hemorrhagic Fever Pesticide formulation which are available in market are only killed the mosquitos but not the larvae. Therefore Production formulation of insecticide as inscticide alternative to eridicate mosquitos and its larvae with agrisol and dodecyl benzen sulfonat as emulsifier nonionic and anionic hydrophilic. The reseach work was aimed to determine the best emulcifier concentrate in pesticide formulation so that the emulsion of oil and water will be stable for a long time. In reseach work the emulsifier concentrate was made, than emulsion stability test between oil and water as well as its effectivity were condected. This formulation could be used as active engridient for insecticide to kill Aedes aigepty mosquitos and its larvae and could be applied in the community. The research showed that formulation consist of komposisi: 17,38% propoxure; Malathion 17,39% agrisol; 13,01% DBS, 47,83 % Xyline dan 4,35% additive dalam % berat. Dengan total emulsifier yang digunakan 30,5 % w/w equivalent 30% w/w. Stability test of oil and water showed that the best solvent is thiner, and formed micro emulsi on water film for more than 12 week. Emulsion was stable by CMC 0,42 % v/v on oil film. From effication test and than analysis profitability revealed that LD 50, was . 0,025 -0,25 % (v/v), both for larvae and mosquitos presented dead 45-90%.
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