Aim: A captive male jaguar (Panthera onca) was anaesthetized for surgical excision of a tumor at the left belly fold under xylazine-ketamine immobilization and propofol anesthesia. The objective was to assess the dose of xylazine and ketamine required to abolish ear flick reflex for safe approach when the jaguar was under chemical immobilization and efficacy of propofol induced anesthesia.Materials and Methods: A male jaguar (P. onca) aged 14 years and weighing approximately 90 kg was subjected to chemical immobilization using a combination of xylazine and ketamine using a blow pipe. The jaguar was approached after the absence of ear flick reflex and transported to zoo Operation Theater. Propofol was administered intravenously to induce and maintain anesthesia. The tumor was excised using thermocautery and subjected to histopathology.Results: Ear flick reflex was stimulated at 5 and 10 min after immobilization and observed shaking of head and movement of fore limb following administration of xylazine and ketamine. Dose of xylazine and ketamine required for chemical immobilization, characterized by absence of ear flick reflex was 1.0 and 3.5 mg/kg body weight respectively, and was achieved in 13 min. The surgical plane of anesthesia was maintained for 11 min following administration of propofol at a dose of 2 mg/kg body weight intravenously. The jaguar recovered in 41 min following surgery. The excised tumor was confirmed as sebaceous adenoma on histopathological examination. The animal recovered uneventfully, and no recurrence of the tumor was noticed in 3 months follow-up period. Conclusion:The total dose xylazine and ketamine required for chemical immobilization with absence of ear flick reflex was 1.0 and 3.5 mg/kg body weight respectively. Further, administration of propofol intravenously, at a dose of 2 mg/kg maintained anesthesia for 11 min. Histopathological examination of the excised tumor at the belly fold was confirmed as sebaceous adenoma.

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| A total of 12 trials were conducted in 8 Asiatic and hybrid lions (Panthera leo persica) for diagnostic and surgical procedures. All the lions were immobilized with a combination of xylazine and ketamine at the rate of 1.00 mg/kg and 2.00 mg/kg body weight, respectively, using darts based on assumed body weight. Ketamine and propofol intravenously were used as induction agents sufficiently to achieve deep plane of anaesthesia and good jaw muscle relaxation in six trials each of treatment I and II. The commercially available large animal endotracheal tubes and custom made silicon medical grade tubes were used for intubation either by direct visualization or by digital palpation of glottis. Anaesthesia was maintained with isoflurane. The study revealed that the young lions required 1.08±0.10 and 2.70±0.26 mg/kg and adult required 1.06±0.30 and 2.64±0.08 mg/kg body weight of xylazine and ketamine, respectively for immobilization. Ear flick reflex was taken as an indicator for safe and appropriate time for approaching the lion after immobilization, which was completely abolished only after 1.37 and 2.01 minutes after recumbency in young and adult lions, respectively. Based on the radiographic distance between snout and distal larynx endotracheal tubes of one meter length and 30 mm diameter were suitable for intubation and the larynx was located between 5 th and 6 th cervical vertebrae. The dose of ketamine and propofol required for induction was 1.00 mg/kg and 1.92 mg/kg body weight, respectively, in immobilized lions and intubation could be safely performed under propofol induction by oropharyngeal digital palpation of glottis for administration of inhalant anaesthetics. Both ketamine and propofol could be used as induction agents in lions immobilized with xylazine-ketamine and for further maintenance with isoflurane.

Aim: Vasectomy is performed in deer for population control, maintain pedigreed animals and prevent inbreeding. Conventional procedure of vasectomy required a long-term anesthesia and longer duration of hospitalization, which often result in stress, morbidity and mortality. A study was conducted to capture, neuter and release the deer with minimal hospitalization and stress by adopting three finger palpation technique of vas deferens and performing vasectomy through a key-hole incision. Materials and Methods:The study was conducted on three spotted male deer and three sambar male deer, which were immobilized with a mixture of xylazine at the dose of 1.00 mg/kg and ketamine at the dose of 5.00 mg/kg. The vas deferens could be palpated as a piece of cooked spaghetti at the neck of the scrotum on the anterior aspect by three finger palpation technique and was able to fix the vas deferens between the thumb and middle finger. Through a key-hole incision of <5 mm length, the vas deferens was exteriorized and resected using electrocautery and the skin incision was sealed with methyl methacrylate. The deer were released on the same day, and no post-operative complication was noticed. Conclusion:The study revealed that three finger palpation technique of vas deferens provided guidance for easy access to vas deferens for vasectomy in deer with less hospitalization, and the deer could be released on the same day.