Two species of entomopathogenic nematodes, an ambush forager (Steinernema carpocapsae) and a cruising forager (S. glaseri), were assayed for their responses to volatiles associated with Galleria mellonella (Insecta: Lepidoptera). The responses of entomopathogenic nematodes to six host-associated volatile treatments were assayed. Live G. mellonella, live G. mellonella with the cuticular hydrocarbons removed, live G. mellonella with carbon dioxide removed, dead G. mellonella, dead G. mellonella with the cuticular hydrocarbons removed, and volatiles from S. exiqua feces were assayed in a Y-tube choice aparatus. Steinernema glaseri were attracted only to treatments producing carbon dioxide. Steinernema carpocapsae showed no significant response to any treatments tested. To assess the directionality of the response toward live G. mellonella, net average movement per nematode after 5, 10, and 20 min was measured for both nematode species. The assay method was based on the "quadrant plate" bioassay, with a 9 cm Petri dish containing 2% agar. Steinernema carpocapsae showed little directionality of response, whereas S. glaseri was attracted to carbon dioxide. We conclude that carbon dioxide attracts S. glaseri to areas likely to hold hosts. Ambush foragers are less influenced by cues associated with hosts or their habitat.
SUMMARYSearch behaviour of two entomopathogenic nematode species with different foraging strategies was compared by measuring parameters of unrewarded search after contact with host cues. Steinernema glaseri cruises in search of hosts. Steinernema carpocapsae ambushes hosts. Nematodes should respond to contact with relevant host cues by shifting their search from ranging to localized after contact with them. We predicted that cruising foragers rely on chemical cues more heavily than ambushers. These species were also tested for host affinities. Nematodes were tracked by image analysis after exposure to faeces, cuticle or food of either Popillia japonica or Spodoptera exiqua. Steinernema glaseri responded to selected host cues by shifting from ranging to localized search, characterized by decreased locomotory rate, distance travelled, search area and the proportion of the test period spent moving. Steinernema carpocapsae did not respond to host cues. Steinernema glaseri responds to selected chemical host cues for host location, whereas S. carpocapsae does not.
ZrC and ZrN ceramics are of interest in the application of materials in extreme high temperature environments, particularly for nuclear applications in generation IV reactors. These materials demonstrate desirable characteristics such as high thermal and electrical conductivities along with high hardness and melting temperatures. Data reported in the literature often suffer from scatter due to differences in processing techniques and difficulty determining stoichiometry, which will significantly affect thermophysical properties. This article reviews the current available data for the properties of ZrC, ZrN and mixed carbonitrides phases and identifies causes of scatter in the literature and areas requiring further research.
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