Summary Besnoitia besnoiti merozoites obtained from cysts in naturally infected bovines and passaged in Vero‐cells were multiplied in cultures of Vero‐ and CRFK‐monolayers. Their development was studied for 7 days p. i. by light and electron microscopy. As early as 24 h p. i. intracellularly situated single parasites were found; 48 h p. i. up to 10 merozoites of Besnoitia were seen in a single parasitophorous vacuole. Irregularly arranged clusters of up to 32 zoites on the 3rd day p. i. changed into regularly formed rosettes of Besnoitia. After the 4th day p. i. the extracellular forms predominated. During asexual multiplication of the parasites plaque formations could be observed 4—5 days p. i. as a cytopathogenic effect, followed 6—7 days p. i. by complete destruction of the monolayers. The predominant type of multiplication with Besnoitia is endodyogeny, which starts by the spherical mother cells developing two merozoite‐anlagen. Forming the daughter cell pellicle, the nucleus of the mother cell shrinks to a large extent and finally divides, the thicker posterior merozoite pole being left open. The young daughter cells remain connected with this pole by a common residual body until the final division takes place. A special form of endodyogeny is the rosette‐formation resulting from several endodyogenies without any separation of the young merozoites from the mother cell. The not yet fully differentiated merozoites of a rosette situated in a parasitophorous vacuole thus induce a mass multiplication of finally well‐developed merozoites of Besnoitia besnoiti. Zusammenfassung Die Ultrastruktur der asexuellen Vermehrung von Besnoitia besnoiti (MAROTEL, 1912) in Vero‐ und CRFK‐Zellkulturen Aus Zysten natürlich infizierter Rinder gewonnene, in Vero‐Zellen passagierte Besnoitia besnoiti‐Merozoiten wurden in Vero‐ und CRFK‐Zellkulturen vermehrt. Ihre Entwicklung konnte 7 Tage lang licht‐ und elektronenmikroskopisch untersucht werden. 24 Std. p. i. traten vereinzelt intrazellulär liegende Parasiten, 48 Std. p.i. bis zu 10 Besnoitia‐Merozoiten in einer gemeinsamen parasitophoren Vakuole auf. Bis zu 32 unregelmäßig zu Haufen angeordnete Zoiten am 3.Tg. p.i. wechselten mit regelmäßig geformten Besnoitia‐Rosetten ab. Nach dem 4.Tg. p.i. traten überwiegend extrazelluläre Parasiten auf. Als zytopathischer Effekt konnte 4—5 Tg. p. i. Plaquebildung, 6—7 Tg. p. i. komplette Zellzerstörung beobachtet werden. Die Endodyo genie stellt den Haupttyp der ungeschlechtlichen Vermehrung von Besnoitia dar, wobei in jeder Mutterzelle zwei Merozoitenanlagen gebildet werden. Von hier aus entwickelt sich die Tochterzellpellikula und gleichzeitig vergrößert und teilt sich der Mutterzellkern. Die jungen Tochterzellen bleiben mit ihrem offenen, verdickten hinteren Pol mit einem gemeinsamen Restkörper bis zur endgültigen Teilung verbunden. Eine besondere Form der Endodyogenie ist die Rosettenbildung, die eine Folge von mehreren Endodyogenien ohne Abtrennung der Merozoiten von der Mutterzelle darstellt. Die noch nicht ausdifferenzierten, als Roset...
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