HIV and malaria co-infections affect iron status. The present study was designed to determine the collective predictive power of some iron markers in HIV infected and malaria co-infected participants. For this study, 101 participants were randomly recruited from indivividuals requesting for HIV screening. The participants were grouped as 'asymptomatic HIV participants (n=36); asymptomatic HIV-malaria co-infected participants (n=19); symptomatic HIV participants (n=16) and HIV uninfected control participants (n=30). Blood analysis were performed for HIV infection, malaria infection, haemoglobin (g/dl), CD4 + T cell count (/mm 3 ), albumin (g/l), iron (ug/dl), UIBC (ug/dl), TIBC (ug/dl) and percent transferrin saturation (TS%). The analysis of variance (ANOVA) showed that the blood concentrations of haemoglobin (f=4.805, p<0.01), iron (f=32.368, p<0.01), TIBC (f= 20.467, p<0.01), TS% (f=7.616, p<0.01) and albumin (f=16.150, p<0.01), were significantly different amongst the groups. The serum albumin, iron, TIBC and TS% were significantly lowered in asymptomatic HIV, asymptomatic HIV-malaria co-infected and symptomatic HIV participants compared respectively with corresponding parameter in the control participants (p<0.01). The CD4 + T cell count was significantly lowered in symptomatic HIV participants compared with asymptomatic HIV participants with or without malaria co-infection (p<0.01). The study suggests that iron status may be affected in HIV infected individuals. The implications of the result are discussed.