Babesia bigemina plays an important role in causing liver and kidney dysfunction in affected animals. The aim of this study evaluating the toxic effect of babesiosis on liver and kidney by measuring biochemical parameters and pathological tissue changes in infected animals with B. Bigemina and chose the best method of treatment. A total of 40 cattle age 1-3 years 30 were suffered from increase in temperatures, off food, Hemoglobin urea, red water from endemic area with babesia. Take sample from infected animals & examine it with microscopic examination found babesia bigemina and confirm by inoculation of heparinized blood from this animals in rats and found rats death at fifth day from infection and histopathological exam found babesia in piroplasma form in pathological tissue of rat's liver & kidney. The infected animals were divided into three groups each group 10 animals untreated group use as control positive and treated groups divided into two groups first group treated with imidocarb only and the second group use imidocarb and lincomcyn as antibiotic drug and compare between two groups by measuring liver& kidney function improvement and compare two groups with negative and positive group which method of treatment more effective in treatment of babesia & decrease toxic effect of babesia.