Two flavonol glycosides; U1: naringenin-7-O-glucoside and U2: kaempferol-3-O-glucoside were isolated for the first time, from ethyl acetate fraction of the stem bark of a traditional medicinal plant called Uapaca heudelotti. IR and NMR spectroscopy were used to elucidate the structures of the isolated compound. The two compounds were active against the 7 tested microorganisms; Escherichia coli, Bacillus subtilis, Salmonella typhi, Streptococcus pyogenes, Klebsiella pneumoniae, Staphylococcus aureus and Proteus mirabilis. The zones of inhibition of the compounds ranged from 16 to 23 mm.The MIC value was as low as 6.25 μg/mL against Salmonella typhi, Streptococcus pyogenes, and Bacillus subtilis. The radical scavenging activity of compound U1 and U2 was 80 and 85 % at 240 μg/mL, while that of the standard drug was 98% at 240 μg/mL. The results show an existent possibility of using the plant for the treatment of microbial diseases.

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Objectives: To isolate, characterize and determine the antimicrobial activity of bioactive compounds present in the petroleum ether fraction of the aerial parts of Aeschynomene uniflora E. Mey. Methods: Aeschynomene uniflora was collected, identified, dried and pulverized. The pulverized plant material was subjected to Soxhlet extraction using petroleum ether. The petroleum ether extract was subjected to chromatographic techniques which yielded J1. J1 was then subjected to antimicrobial screening using some selected microorganisms. J1 was characterized using IR and NMR spectroscopic techniques. Results: The structure of the isolated compound was established to be 3β, 22E-Stigmasta-5, 22dien-3-ol (stigmasterol) using spectroscopic analysis (IR, 1D-NMR). The isolated compound showed significant antimicrobial activity on some selected microorganisms. Conclusion:The results could justify the use of the aerial parts of Aeschynomene uniflora in traditional herbal medicine for the treatment of various microbial diseases.

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