The perception of affect influences the subjective perception of an individual's environment (Isbell & Burns, n.d.). Accurate affect perception leads to increased resilience and positive coping mechanisms when faced with daily life stressors (Robinson, Moeller, Buchholz, Boyd, & Troop-Gordon, 2012). Communication technologies have revolutionized the ways in which individuals connect to one another professionally and socially (Joseph B. Walther, 1992). This study investigated accurate affect perception in computer mediated communication (CMC) from a multidisciplinary perspective. One hundred fifty four research participants (= 26.84, = 9.66) responded to a self-report questionnaire hosted by Qualtrics.com. A multiple linear regression was conducted, regressing accurate affect perception in the CMC environment on personality, mood, demographic information, and attitude toward CMC variables. The analysis was significant, R 2 = .2, F(13,140) = 2.60, p = .003. and lends significant support for previous research in the cognitive neuroscience field that posits positive mood is the most significant predictor of affect perception, β = .12, t(13) = 2.52, p = .008. Proposed methods to increase ones affect perception in CMC and future research directions are discussed.