Potential risk of allergenic, toxic, and dietary risks from Genetically Modified Organism (GMO) has become critical issues. The transgenic soybean of United States has commonly been exported to countries around the world, including Indonesia. Unfortunately, there has not yet been any label given to packaging product of Genetically Modified Organism (GMO) including soybean grain sold in Indonesia. The aim of the study was to detect promoter of Cauliflower Mozaic Virus 35 S which potentially indicate the transgenic plants. Samples used in this research were 12 different brands of soybean sold in the 4 local markets. Screening of CaMV 35 S genes was done by Polymerase Chain Reaction (PCR) methods using specific primers. The result indicated that positive signals detection refers to transgenic plants in the local soybean grains with the amplification area of 123 basepairs.
Fifty percents of soybean in Indonesia has been imported from the United States. Most of 94 % imported soybean was genetically engineered soybean. In meanwhile, products of Genetically Modified Organism (GMO) of Indonesia were not labeled yet in the market packaging products. This aim of the study study was to detect EPSPS-CP4 gene which potentially indicates of transgenic gen of soybean in order to help Indonesia to implement government regulation on label and food advertisement and fulfill consumer right. The result indicated that the local soybean also contained Genetically Engineered (GE) herbicide-tolerant gene called EPSPS-CP4 gene of transgenic soybean by using Polymerase Chain Reaction (PCR) method. Those Local soybeans brand name sold in the market were Lokal, Galunggung, and Wonosari.
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