Objective: To evaluate the effect of pasteurization on antioxidant and oxidant properties of human milk. Methods: 42 samples of milk before and after pasteurisation were used to evaluate the antioxidant activity by the ferric reducing capacity and by scavenging the 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical. Lipid peroxidation was estimated by the concentration of malondialdehyde product using the thiobarbituric acid reactive substances assay and by the evaluation of advanced oxidation protein products. Results: No significant difference was observed in fresh human milk and after pasteurization in relation to antioxidant properties determined by the ferric reducing capacity (50.0±3.4% and 48.8±3.0%, respectively) and by scavenging the 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid radical (28.9±1.5% and 31.2±1.3%, respectively). The results of malondialdehyde (62.6±4.1 and 64.3±3.6 µM/mg) and protein oxidation products (59.4±3.4 and 54.2±3.8 µM/L) of fresh and pasteurized milk, respectively, did not exhibited any significant difference. Conclusions: This data showed that human milk has an important antioxidant activity and that the pasteurizing process does not influence the antioxidant capacity, avoiding the peroxidation of breast milk lipids and the formation of advanced protein oxidation products.

Background: Human milk is an essential source of nutrition for an infant’s health. When breastfeeding working mothers or students, for example, are unable to breastfeed, storing their milk is recommended. Therefore, it is crucial to know the storage conditions to ensure their antioxidant capacity and avoid oxidative damage. Research Aim: To evaluate the stability of the antioxidant and pro-oxidant profiles and the amount of total protein in fresh human milk after different storage times (1, 2, 7, 14, and 21 days) and temperatures (4 ºC and -20 ºC). Methods: This was a prospective, longitudinal, and observational study with milk samples grouped according to age for comparisons, which included 20 lactating women. The antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid and the decrease of ferric ion. Oxidative stress was determined by the lipid peroxidation product formation through malondialdehyde concentration, and the total protein content was assessed by the Bradford method. Results: The antioxidant profile of human milk was maintained with minimal losses until the 14th day when stored at 4 ºC and -20 ºC. The evolution of malondialdehyde concentration over storage revealed significant changes only 21 days after human milk storage at 4 ºC. There was no change in the value of total protein content. Conclusions: To sum up, there is no difference in the storage of human milk at a temperature of 4 °C or -20 °C over 14 days. Therefore, the lactating woman may choose the most convenient way of storage.

A terapia assistida por animais (TAA) é utilizada como instrumento no tratamento de doenças, com diversos efeitos benéficos em paciente psiquiátricos, hospitalizados, idosos, dentre outros. A TAA objetiva promover o bem-estar, saúde emocional, física, social e cognitiva, sendo o animal o principal agente da terapia, que funciona como ponte de ligação entre o tratamento e o paciente. A institucionalização do idoso ocorre, geralmente, após ocorrência de alterações físicas, cognitivas, doenças agudas ou crônicas, perda do cônjuge ou filho, perda de apoios sociais, pobreza, gerando dependência na realização das atividades da vida diária e locomoção. Trata-se de estudo clínico experimental controlado, realizado em uma casa de repouso em Vila Velha, ES, com o objetivo de avaliar a influencia da TAA na pressão arterial de idosos hipertensos institucionalizados. Participaram do estudo 25 idosos. As variáveis utilizadas no trabalho foram TAA (independente), variação de Pressão Arterial (dependente) e idade (controle). Foram realizadas sessões de TAA semanalmente com a duração de 1 hora e a pressão arterial foi aferida com uso de esfigmomanômetro antes e após a cada sessão durante 4 meses. Observou-se melhor controle dos níveis pressóricos além da promoção de momentos de alegria e relaxamento da amostra representativa do grupo de idosos institucionalizados.

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Plants have been used for centuries as therapeutic resources for various pathological conditions and are occasionally considered as the only resource available to some communities. However, knowledge on the potential of traditional medicinal plants have been lost over the years. Strategies that can guarantee the prospection of popular knowledge regarding medicinal plants to stimulate the spread of information between generations are therefore of enormous value. Thus, the present study aimed to investigate the use and knowledge on medicinal plants of students from a public school in Vila Velha (Brazil) and their associated community, including parents. In this urban locality, data were obtained by the application of a semi-structured questionnaire to 206 people from the school community. Although homegrown cultivation seems to be an uncommon practice in urban contexts, the use of medicinal plants by the sampled population was significant (80%), as well as the interviewees' confidence on its therapeutic action (90%). It is worth mentioning that more than 70% of the respondents presented their parents and/or grandparents as their main source of information, highlighting the importance of popular knowledge valorization.