The decreasing productivity and increasing environmental pressure on intensive tilapia culture in open waters, especially in floating net cages, have forced the development of a biofloc system for tilapia farming. This study was aimed to develop the superior tilapia strain suitable for the biofloc system, their productivity and economic profitability under commercial scale in the biofloc system. The selective breeding activity was carried out on 100 families reared and mated in hapa in the biofloc pond for three generations. Individual selection was conducted at the age of 6 months on the characters of standard length and body weight with the best 10% reference for formation of the next generation. After three generations, the offspring obtained from mass spawning were grown out in round-limen ponds and square-concrete ponds for productivity and profitability observations. The results showed that heritability values ​​of the biofloc tilapia strain were 0.4196 (standard length, SL) and 0.4898 (body weight, BW). The average response selection of BW and SL were 13.04% and 6.11%, respectively. Biomass gain was obtained between 19.29–28.83 kg/m3. The production multiply value was 8.07-13.59x. The average daily weight growth was between 2.8g-3.0g. The Benefit-Cost ratio valued from 1.81 to 2.71.