In the present study, a longitudinal assessment was carried out to evaluate the short-term human exposure to aflatoxins in Pirassununga region, São Paulo, Brazil, by determination of urinary aflatoxins by a liquid chromatography coupled to mass sprectrometry (UPLC-MS/MS) method. Sixteen volunteers with ages ranging from 14 to 55 years old were instructed to collect the early morning first urine four times every three months, from June 2011 to March 2012, totaling 64 samples. Aflatoxin M1 (AFM1) was found in 39 samples (61%) at levels ranging from 0.19 to 12.7 pg·mg−1 creatinine (mean: 1.2 ± 2.0 pg·mg−1 creatinine). Residues of aflatoxins B1, B2, G1, G2 and aflatoxicol were not identified in any urine sample. No significant difference was found among the AFM1 mean levels in urine samples collected in the four sampling periods. The levels of AFM1 found in urine samples indicate a low short-term exposure of the population studied to aflatoxins through the diet, although further investigations are needed to assess other long-term biomarkers of exposure to AFB1.

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pela confiança e orientação. A Profa. Dra. Ana Maria Centola Vidal pelo empréstimo do laboratório, orientação, auxilio no decorrer da pesquisa, conselhos e carinho. Ao Prof. Dr. Júlio César de Carvalho Balieiro pela assistência nas análises estatísticas. À querida técnica de laboratório Dra. Andréia Cristina Nakashima Vaz por sua amizade, ensinamentos, carinho e auxilio nas análises laboratoriais. Aos meus (as) estagiários (as) Gustavo, Ângela, Caroline e Ariana pela ajuda, animação e dedicação no decorrer das análises de laboratório e fabricação dos queijos. À colega Natália Bertini pelo companheirismo e carinho a mim concedido. Ao Rafael, Rose, Rosilda e João pela facilitação e auxílio nas análises laboratoriais. Aos funcionários (as) do laticínio do campus "Fernando Costa" pela paciência e ajuda na fabricação dos queijos. Ao meu noivo Me. Marcelo Candido pelo carinho, а paciência, o companheirismo, os ensinamentos е pоr sua capacidade dе me trazer pаz. A minha mãe Zélia e meu pai José Luis Privatti por todo amor e pelo apoio em minha escolha e serem muito importantes em minha vida. Aos meus avôs e demais familiares pelo carinho e apoio. À Capes pela concessão da bolsa de mestrado, o qual me proporcionou me dedicar exclusivamente nessa pesquisa. Aos professores da Faculdade de Zootecnia e Engenharia de Alimentos (FZEA/USP-Pirassununga, SP) pelos ensinamentos e contribuições. A todos aqueles que contribuíram de alguma forma para a realização deste trabalho os meus sinceros agradecimentos. RESUMO PRIVATTI, R. T. Efeitos da contagem bacteriana total do leite cru refrigerado sobre os parâmetros físico-químicos, microbiológicos e rendimento durante a vida útil do queijo Minas Frescal. 2017. 86f. Dissertação

The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.