Caries is an infectious diseases of the oral cavity in which oral biofilms play a causative role. Moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and drugs acting on biofilm to, due to their widespread presence and accessibility. The aim of the present in vitro study was to investigate the efficacy of specific fluoridated toothpaste on the biofilms formed by the test microorganisms (Streptococcus mutans, Facklamia homins and Streptococcus saliviurus). The colonizations of the three test bacteria on four surfaces (two natural; permenant and deciduous tooth surfaces and two artificial ;composite and amalgam filling surfaces) were observed with scanning electron microscopy (SEM).SEM detected the anti-biofilm effects on the same four surfaces medicated with a tooth paste. Results: The used tooth paste declined the formed biofilms on four surfaces efficiently. The natural tooth surfaces responded in same manner to the tooth paste while composite responded more efficiently than amlagam surfaces. Conclusions: Flourdated toothpaste (0.454%) effectively can remove formed biofilms of the three test bacteria on natural surfaces and composite filling surfaces. It removed formed biofilm on amalgam surfaces but not as from other three surfaces.