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Rice bran protein (RBP) was utilized to prepare bio-composite films after deamidation modification process with deferent percentages. The physical, mechanical properties, exposure to light properties, FT-IR analysis, surface shape by SEM, thermal stability by DSC and X-ray diffraction were applied to study the potential interaction, structure and stabilizing the thermal property of the prepared films. The deamidation process has proven successfully, depending on the cleared changes of shapes of FT-IR curves resulting from RBP compared with deamidated rice bran protein (DRBP). The deamidation process enhanced tensile strength, elongation at break, haze, transparency, gloss and opacity properties of produced films from DRBP. Additionally, the produced films from DRBP showed smooth and homogenous surface with increasement of thickness, solubility and decrement of water vapor permeability and crystallinity compared with films from RBP.