Purpose: This is an in vitro study for comparative evaluation of transverse bond strength, peel bond strength and water sorption properties between heat polymerized and auto-polymerized resins. Materials and methods:Two denture soft liners (Molloplast-B, Coe-Soft) were investigated. A total number of 60 specimens were used. Twenty specimens were used for evaluation of each physical property of soft liner. For transverse bond strength, ten specimens (64 × 2.5 × 10 mm) of each liner were made by processing the denture liners with heat cure polymethyl methacrylate (Meliodent). All specimens for transverse bond strength were conducted on samples immersed in distilled water at 37°C for 50 hours by using three-point transverse flexural tests in a Lloyd's Universal Testing Machine and transverse bond strength was calculated based on the maximum load, span length, breadth and thickness. Another set of ten specimens of (75 × 25 × 3 mm) of each denture liner were bonded over 25 mm of heat cure PMMA and separated over the remaining 50 mm of acrylic plate to estimate the peel bond strength. The peel bond strength were conducted on specimens which were dried at room temperature for 48 hours and tested at 21 ± 1°C in a Lloyd's testing machine that was linked to an IBM Compatible Computer. The specimens were deformed with a cross head speed of 5 mm/minute according to ASTMD-903 and peel bond strength was calculated when peel angle was 180°. For water sorption tests, ten disc shaped samples of each liner of 50 mm in diameter and 0.5 mm in thickness were fabricated. After polymerization of the liners, the samples were then stored in distilled water at 37°C for 7 days. After one week, excess moisture was removed and each sample was weighed using electronic weighing machine. Results:The transverse bond strength of Molloplast-B and Coe-Soft were almost similar and did not show any significant difference in their values. But, there is a significant decrease in the peel bond strength and water sorption property of Molloplast-B and Coe-Soft. Conclusion:The reason for transverse bond strength results being similar for both the liners is because they were polymerized chemically with the denture base resins and this chemical affinity could have made its bond strength almost equal. Since, there is a decrease in the peel bond strength of Molloplast-B, the chances of stripping of the liner at the flanges of the denture is minimal. The decrease in water sorption of Molloplast-B can be expected to retain the bond with the denture and sustain the resilience property of the material for a longer time.