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This study was conducted in Diyala province from1 Nov. 2016 to April 1, 2017) and included (200) blood samples were used serological tests for diagnosis in the laboratories of Baquba General Teaching Hospital, Blood Bank Department for prevalence of infection by bacteria Salmonella typhi and Brucella melitensis among blood donors at the Central Blood Bank . The study showed that the prevalence of Salmonella spp and Brucella in Diyala (69%) and (31%) respectively.The study also showed that the age group (31–40) is the most susceptible to bacterial infection also the prevalence of salmonella in the city is higher than the village where the incidence of Salmonella in the city (73.33%) and in the village (26.67%) . The proportion of infection with Brucella melitensis in the city (69%) and in the village (30%).

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The study group included 100 blood samples pre-marital women in Diyala / Al-Khalis, form the period from 1/10/2015 to 1/4/2016, the blood sample of women without any clinical evidence of cytomegalovirus (CMV) infection and free from infected by HIV .we were screened for the presence of IgG and IgM antibodies against CMV by strip test.The IgG antibodies were detected in 10%, while the IgM antibodies were detected in (6%). The sero negative (control) was (90%) for IgG and (94%) for IgM. Rising in seropositivity was observed in young women in age group (25-30years) for both IgG and IgM, Seroprevalence of CMV was more in women from urban area IgG (80%), IgM(83%) with no significant result. Most of the women have higher education rising to 90% for IgG and 100% for IgM.