Matrix type transdermal patches containing Glibenclamide were prepared using three different polymers by solvent evaporation technique. Aluminium foil cup method was used as a substrate. Polyethylene glycol (PEG) 400 was used as plasticizer and Dimethyl sulfoxide (DMSO) was used as penetration enhancer. The physicochemical parameters like weight variation, thickness, folding endurance, drug content, % moisture absorption and % moisture loss were evaluated. In vitro drug release studies and skin permeation studies were carried out using Franz diffusion cell. Cumulative amount of drug released in 12 hours from the six formulations were 55.467, 52.633, 47.157, 53.394, 49.139 and 45.597 %, respectively. The corresponding values for cumulative amount of drug permeated for the said formulation were 43.013, 40.429, 37.793, 41.522, 37.450 and 34.656 %, respectively. On the basis of in vitro drug release and skin permeation performance, formulation HP-1 was found to be better than other formulations and it was selected as the optimized formulation.

T he purpose of this study was to construct a microemulsion-based hydrogel formulation for the transdermal delivery of dexamethasone. Almond oil, olive oil, linseed oil, and nutmeg oil were screened as the oil phase. A microemulsionbased system was chosen due to its good solubilizing capacity and skin permeation capabilities. The pseudoternary phase diagrams for microemulsion regions were constructed using various oils, egg lecithin as the surfactant, isopropyl alcohol (IPA) as the cosurfactant, and distilled water as the aqueous phase. Microemulsion gel formulations were prepared using Carbopol and filled into a reservoir-type transdermal system. The ability of various microemulsion formulations to deliver dexamethasone through the rat skin was evaluated in vitro using Keshary Chien diffusion cells. In order to enhance permeation, the skin was treated with an abrading gel (apricot seed powder in hydrogel base). The in vitro permeation data showed that microemulsions increased the permeation rate of dexamethasone compared with the control. The optimum formulation consisting of 0.1% dexamethasone, 10% olive oil, 70% egg lecithin:IPA (2:1), and water showed a permeation rate of 54.9 µg/cm 2 /h. The studied microemulsion-based hydrogel was stable toward centrifugation test and was nonirritating to the skin. The pharmacodynamic studies indicated that microemulsion based on nutmeg oil demonstrated a significantly (P < 0.05) higher anti-inflammatory potential. The nutmeg oil-based transdermal microemulsion gel system demonstrated 73.6% inhibition in rat paw edema. Thus, microemulsion-based transdermal systems are a promising formulation for dermal delivery of dexamethasone.

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Medicinal plants are one of the oldest valuable sources of medicine provide by nature to mankind. Medicinal plants are one of the important sources of research of new drugs. Many medicinal plants and herbs hold their prestigious position in the field of natural medicine among which Anogeissus acuminata belonging to family Combretaceae was vital one. In the present study was intended to evaluate various physicochemical, phytochemical standards like TLC, HPTLC and Pharmacognostic parameters which will be helpful to ensure the purity, safety and efficacy of the medicinal plant.  Anogeissus acuminata was subjected to preliminary phytochemical screening, done to estimate as foreign organic matter, morphological evaluation. Total ash values, physicochemical, Thin layer chromatography (TLC), HPTLC phytochemical analysis and secondary metabolites present in the plant were investigated for the extract. All physiochemical properties were found within the measureable amount as foreign organic matter less than 1% w/w, Total ash values was 12 % w/w with respect to air-dried crude drug. Moisture content was 3.074. Thin layer chromatography (TLC), HPTLC studies were carried for the better isolation and identification of the different components of ethanolic extract. HPTLC carried out in the solvent system - Toluene: Ethyl acetate: Acetic acid (4:3:1). The report of HPTLC indicates the presence of sixteen spots. Preliminary phytochemical analysis confirmed the presence of plant extracts revealed the presence of alkaloids, steroids, terpenoids glycosides, tannins and phenolic compounds. KEYWORDS: TLC, HPTLC, Phytochemical characterization, Anogeissus acuminata