Mikroplastik (MP) telah ditemukan di berbagai lingkungan laut khususnya pada ikan, dan informasi terkait akumulasi mikroplastik pada organ-organ ikan masih sangat terbatas. Penelitian ini bertujuan untuk membuktikan keberadaan mikroplastik pada organ insang, saluran pencernaan, dan daging ikan ekonomis penting (Hemiramphus far, Siganus virgatus, dan Lethrinus lentjan) di perairan Pulau Barranglompo, Makassar, serta mengidentifikasi karakteristik mikroplastik meliputi warna, bentuk, dan ukuran. Setiap organ diekstraksi menggunakan KOH 20% dan pengamatan karakteristik MP dilakukan secara visual menggunakan mikroskop stereo. Hasil penelitian menunjukkan bahwa mikroplastik ditemukan di insang, saluran pencernaan, dan daging dari ketiga spesies ikan. Tingkat deteksi mikroplastik pada masing-masing H. far, S. virgatus, dan L. lentjan adalah 100%, 100%, dan 82%. Karakteristik warna mikroplastik didominasi oleh biru, bentuk mikroplastik didominasi oleh line, dan kisaran ukuran mikroplastik didominasi 1–5 mm. Penelitian lebih lanjut perlu dilakukan untuk melihat akumulasi mikroplastik pada setiap organ secara eksperimental di laboratorium.
High levels of anthropogenic activities on Barranglompo Island in Makassar City, South Sulawesi Province, Indonesia increase the amount of plastic waste in the surrounding waters, especially in seagrass beds. Plastic waste becomes fragmented into small particles called microplastics (MPs). Sea urchins (Echinoidea), as organisms associated with seagrass beds, can accumulate microplastics through their feeding habits. This study aimed to determine the abundance and characteristics of microplastics accumulated in sea urchins (Diadema setosum), seagrass leaves, and sediment. Samples (urchins, seagrass leaves, and sediment) were collected from a multi-species (Enhalus acoroides and Thalassia hemprichii) seagrass bed where D. setosum was present. The internal organs of the sea urchins were extracted using a KOH 10% solution to degrade organic materials. The seagrass leaves were rinsed using distilled water and stirred using a shaker rotator. MPs in sediment were separated using ZnBr2. MPs in all samples were identified visually under a stereomicroscope. The results show that all (100%) sea urchins samples (n=10), seagrass leaves (n=24), and sediment samples (n=8) were contaminated by MPs. The average MP abundance was 23.70±2.99 items/individual in D. setosum; 0.10±0.02 items/cm2 and 0.24±0.05 items/cm2 respectively on E. acoroides and T. hemprichii leaves; and 195±66.98 items/kg of dry weight in the sediment. The MPs found shared similar characteristics such as colour (predominantly blue) and shape (dominated by line).
HighlightIdentification and morphological analysis of Selaroides leptolepis fish.Molecular analysis using the DNA Barcoding method with the COI gene (mitochondrial cytochrome oxidase subunit I).The number of base pairs in S. leptolepis from the sequencing results was 675bp.The morphological and molecular similarities of Selaroides leptolepis fishAbstractYellowstripe scad is one of the commercially important fish from the Carangidae family, which is marketed at Muara Baru Modern Fish Market, North Jakarta. In- formation regarding the presence of Selaroides leptolepis fish in the waters allows for effective conservation, and management of marine resources. A morphological identification of fish species is still considered inaccurate, so the molecular anal- ysis is necessary. This study aims to identify commercially important fish species deriving from Muara Baru Modern Fish Market, North Jakarta, employing mor- phological, and molecular analysis. A total of 30 specimens were collected from the field. The morphological analysis utilized a visual identification method, and morphometric measurement, while molecular analysis with DNA barcoding em- ployed the mitochondrial cytochrome oxidase subunit I (COI) gene. The results of the morphological analysis indicated that the fish species were yellowstripe scad (S. leptolepis). It also followed the results of molecular analysis of DNA barcoding that the fish was a S. leptolepis species. Therefore, the combination of morphological and genetic analysis has succeeded in identifying the fish species of S. leptolepis.
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