Conjugative plasmids are typically locked in intergenomic and sexual conflicts with coresident rivals, whose translocation they block using fertility inhibition factors (FINs). We describe here the first crystal structure of an enigmatic FIN Osa deployed by the proteobacterial plasmid pSa. Osa contains a catalytically active version of the ParB/Sulfiredoxin fold with both ATPase and DNase activity, the latter being regulated by an ATP-dependent switch. Using the Agrobacterium tumefaciens VirB/D4 type-IV secretion system (T4SS), a relative of the conjugative T4SS, we demonstrate that catalytically active Osa blocks T-DNA transfer into plants. With a partially reconstituted T4SS in vitro, we show that Osa degrades T-DNA in the T-DNA-VirD2 complex prior to its translocation. Further, we present evidence for conservation and interplay between ATPase and DNase activities throughout the ParB/Sulfiredoxin fold, using other members of the family, namely P1 ParB and RK2 KorB, which have general functional implications across diverse biological contexts.

In this paper, we propose four continuous authentication designs by using the characteristics of arm movements while individuals walk. The first design uses acceleration of arms captured by a smartwatch's accelerometer sensor, the second design uses the rotation of arms captured by a smartwatch's gyroscope sensor, third uses the fusion of both acceleration and rotation at the featurelevel and fourth uses the fusion at score-level. Each of these designs is implemented by using four classifiers, namely, k nearest neighbors (k-NN) with Euclidean distance, Logistic Regression, Multilayer Perceptrons, and Random Forest resulting in a total of sixteen authentication mechanisms. These authentication mechanisms are tested under three different environments, namely an intra-session, inter-session on a dataset of 40 users and an inter-phase on a dataset of 12 users. The sessions of data collection were separated by at least ten minutes, whereas the phases of data collection were separated by at least three months. Under the intra-session environment, all of the twelve authentication mechanisms achieve a mean dynamic false accept rate (DFAR) of 0% and dynamic false reject rate (DFRR) of 0%. For the inter-session environment, feature level fusion-based design with classifier k-NN achieves the best error rates that are a mean DFAR of 2.2% and DFRR of 4.2%. The DFAR and DFRR increased from 5.68% and 4.23% to 15.03% and 14.62% respectively when feature level fusion-based design with classifier k-NN was tested under the inter-phase environment on a dataset of 12 users.

IntroductionOmentum can secrete out biological agents like different growth factors, cytokines, and antimicrobial peptides. The aim of our study was to determine the expression of antimicrobial peptides and cytokines in human omentum tissue and its response to intra-abdominal infection. MethodologyOmentum tissue was obtained from 60 patients: control (n=20) and cases (n=40). mRNA expression of antimicrobial peptides (LL-37, HBD-1, HBD-2, HNP1-3) and cytokines (TNFα, IL-8, IL-10, IL1β) was evaluated using Real-Time PCR. Protein quantification was done by Immunoblotting and ELISA. ResultsSignificantly higher expression of antimicrobial peptides (LL-37, HBD-1, HBD-2, HNP1-3) and cytokines (TNFα, IL-8, IL-10, IL1β) was observed in cases as compared to control at both the transcriptional and translational level (p<0.0001). ConclusionOmentum governs a population of antimicrobial peptides with potent immunologic functions. The expression of antimicrobial peptides and cytokines is inducible and increases with the severity of infection. Omentum is thus an immunologically active and adaptable organ but its complete regulatory mechanism is still elusive.

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