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<b><i>Background:</i></b> Gamma-aminobutyric acid (GABA)ergic and opioid systems play a crucial role in the neural modulation of innate fear organised by the inferior colliculus (IC). In addition, the IC is rich in GABAergic fibres and opioid neurons, which are also connected to other mesencephalic structures, such as the superior colliculus and the substantia nigra. However, the contribution of distinct opioid receptors (ORs) in the IC during the elaboration and expression of innate fear and panic-like responses is unclear. The purpose of the present work was to investigate a possible integrated action exerted by ORs and the GABA_A receptor-mediated system in the IC on panic-like responses. <b><i>Methods:</i></b> The effect of the blockade of either µ₁- or κ-ORs in the IC was evaluated in the unconditioned fear-induced responses elicited by GABA_A antagonism with bicuculline. Microinjections of naloxonazine, a µ₁-OR antagonist, or nor-binaltorphimine (nor-BNI), a κ-OR antagonist, were made into the IC, followed by intramesencephalic administration of the GABA_A-receptor antagonist bicuculline. The defensive behaviours elicited by the treatments in the IC were quantitatively analysed, recording the number of escapes expressed as running (crossing), jumps, and rotations, over a 30-min period in a circular arena. The exploratory behaviour of rearing was also recorded. <b><i>Results:</i></b> GABA_A-receptor blockade with bicuculline in the IC increased defensive behaviours. However, pretreatment of the IC with higher doses (5 µg) of naloxonazine or nor-BNI followed by bicuculline resulted in a significant decrease in unconditioned fear-induced responses. <b><i>Conclusions:</i></b> These findings suggest a role played by µ₁- and κ-OR-containing connexions and GABA_A receptor-mediated neurotransmission on the organisation of panic attack-related responses elaborated by the IC neurons.

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Spatial cognition is related to academic achievement in science, technology, engineering, and mathematics (STEM) domains. Neuroimaging studies suggest that brain regions’ activation might be related to the general cognitive effort while solving mental rotation tasks (MRT). In this study, we evaluate the mental effort of children performing MRT tasks by measuring brain activation and pupil dilation. We use functional near-infrared spectroscopy (fNIRS) concurrently to collect brain hemodynamic responses from children’s prefrontal cortex (PFC) and an Eye-tracking system to measure pupil dilation during MRT. Thirty-two healthy students aged 9–11 participated in this experiment. Behavioral measurements such as task performance on geometry problem-solving tests and MRT scores were also collected. The results were significant positive correlations between the children’s MRT and geometry problem-solving test scores. There are also significant positive correlations between dorsolateral PFC (dlPFC) hemodynamic signals and visuospatial task performances (MRT and geometry problem-solving scores). Moreover, we found significant activation in the amplitude of deoxy-Hb variation on the dlPFC and that pupil diameter increased during the MRT, suggesting that both physiological responses are related to mental effort processes during the visuospatial task. Our findings indicate that children with more mental effort under the task performed better. The multimodal approach to monitoring students’ mental effort can be of great interest in providing objective feedback on cognitive resource conditions and advancing our comprehension of the neural mechanisms that underlie cognitive effort. Hence, the ability to detect two distinct mental states of rest or activation of children during the MRT could eventually lead to an application for investigating the visuospatial skills of young students using naturalistic educational paradigms.